PMID- 10209339
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 440
IP  - 2
DP  - 1999 Apr 6
TI  - Low persistence of radiation-induced centromere positive and negative micronuclei 
      in cultured human cells.
PG  - 163-9
AB  - The micronucleus (MN) assay is widely used both in genetic toxicology and in the 
      biomonitoring of human populations. Lymphocytes, cell lines, and bone marrow and 
      epithelial cells are usually employed as target systems in such studies. However, 
      little effort has been done to assess the persistence of MN in highly 
      proliferative cells. To study the behaviour of MN containing whole chromosomes or 
      acentric fragments, we have performed a time course experiment on the persistence 
      of gamma-ray (3 Gy) induced MN in a human lymphoblastoid cell line. The frequency 
      and content of MN were analyzed 1, 3, 7, 14, and 56 days after irradiation by 
      pancentromeric fluorescence in situ hybridization (FISH). We observed a clear 
      induction of both centromere positive and negative MN at completion of the first 
      mitotic division. The frequency of both types of MN drastically declined to basal 
      levels 7 days after irradiation with an identical kinetics. We therefore conclude 
      that centromere positive and negative MN are highly unstable upon cell division, 
      indicating that the MN assay could not be a good biomarker of DNA damage induced 
      by acute treatments in highly proliferative cells. The implication of our 
      findings in biomonitoring and in genotoxicity studies is discussed.
CI  - Copyright 1999 Elsevier Science B. V.
FAU - Ramirez, M J
AU  - Ramirez MJ
AD  - Grup de Mutagenesi, Unitat de Genetica, Departament de Genetica i de 
      Microbiologia, Edifici Cn, Universitat Autonoma de Barcelona, 08193 Bellaterra, 
      Cerdanyola del Valles, Spain.
FAU - Surralles, J
AU  - Surralles J
FAU - Puerto, S
AU  - Puerto S
FAU - Creus, A
AU  - Creus A
FAU - Marcos, R
AU  - Marcos R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Biomarkers)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Biomarkers
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - Centromere/genetics/*radiation effects
MH  - DNA Damage/genetics
MH  - Gamma Rays/*adverse effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Micronuclei, Chromosome-Defective/genetics/*radiation effects
MH  - Time Factors
EDAT- 1999/04/21 00:00
MHDA- 1999/04/21 00:01
CRDT- 1999/04/21 00:00
PHST- 1999/04/21 00:00 [pubmed]
PHST- 1999/04/21 00:01 [medline]
PHST- 1999/04/21 00:00 [entrez]
AID - S1383-5718(99)00023-6 [pii]
AID - 10.1016/s1383-5718(99)00023-6 [doi]
PST - ppublish
SO  - Mutat Res. 1999 Apr 6;440(2):163-9. doi: 10.1016/s1383-5718(99)00023-6.