PMID- 10209485
OWN - NLM
STAT- MEDLINE
DCOM- 19990426
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 186
IP  - 4
DP  - 1998 Dec
TI  - Monocyte chemoattractant protein-1 (MCP-1) expression in primary 
      lymphoepithelioma-like carcinomas (LELCs) of the lung.
PG  - 372-7
AB  - Lymphoepithelioma-like carcinoma (LELC) of the lung is a recently recognized 
      primary non-small cell lung carcinoma with distinct clinicopathological features 
      and an aetiological association with Epstein-Barr virus (EBV) infection. The 
      tumour consists of clusters and sheets of poorly or undifferentiated tumour cells 
      in close association with numerous mononuclear inflammatory cells, including a 
      rich component of tumour-associated macrophages (TAMs). To investigate the 
      molecular mechanism leading to the TAM-rich stroma, the expression of a 
      monocyte-specific chemotactic and activating factor, monocyte chemoattractant 
      protein-1 (MCP-1), was studied by reverse transcriptase-polymerase chain reaction 
      (RT-PCR) and in situ hybridization (ISH), and the presence of TAMs was 
      demonstrated by immunohistochemistry in nine LELCs. The results were compared 
      with those found in 17 conventional non-small cell lung carcinomas. RT-PCR showed 
      specific MCP-1 amplification in both LELCs and non-LELCs, but ISH demonstrated a 
      unique and extensive expression of MCP-1 transcripts by the tumour cells of LELCs 
      only, while TAMs, stromal fibroblasts, and endothelial cells formed the major 
      source of MCP-1 in non-LELCs. TAMs in LELCs were more abundant and showed a close 
      topographical relationship with the MCP-1-expressing tumour cells. The results 
      indicate that tumour cell expression of MCP-1 in LELCs is an important mechanism 
      contributing to their distinctive morphological features. This is the first study 
      that demonstrates the in vivo upregulation of a monocyte-specific chemokine by 
      EBV-related carcinomas, illustrating an interesting aspect of tumour biology in 
      EBV-related neoplasms.
FAU - Wong, M P
AU  - Wong MP
AD  - Department of Pathology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
FAU - Cheung, K N
AU  - Cheung KN
FAU - Yuen, S T
AU  - Yuen ST
FAU - Fu, K H
AU  - Fu KH
FAU - Chan, A S
AU  - Chan AS
FAU - Leung, S Y
AU  - Leung SY
FAU - Chung, L P
AU  - Chung LP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Chemokine CCL2)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*metabolism/pathology/virology
MH  - Chemokine CCL2/*metabolism
MH  - Epstein-Barr Virus Infections/complications
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization
MH  - Lung Neoplasms/*metabolism/pathology/virology
MH  - Macrophages/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Proteins/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/04/21 02:02
MHDA- 2000/06/20 09:00
CRDT- 1999/04/21 02:02
PHST- 1999/04/21 02:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/04/21 02:02 [entrez]
AID - 10.1002/(SICI)1096-9896(199812)186:4<372::AID-PATH204>3.0.CO;2-8 [pii]
AID - 10.1002/(SICI)1096-9896(199812)186:4<372::AID-PATH204>3.0.CO;2-8 [doi]
PST - ppublish
SO  - J Pathol. 1998 Dec;186(4):372-7. doi: 
      10.1002/(SICI)1096-9896(199812)186:4<372::AID-PATH204>3.0.CO;2-8.