PMID- 10211110
OWN - NLM
STAT- MEDLINE
DCOM- 19990426
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 186
IP  - 3
DP  - 1998 Nov
TI  - Fluorescence in situ hybridization analysis of chromosome 12 anomalies in semen 
      cells from patients with carcinoma in situ of the testis.
PG  - 235-9
AB  - Carcinoma in situ (CIS) of the testis is the precursor of seminomas and 
      non-seminomatous germ cell tumours of the adult testis. A marked cytogenetic 
      anomaly, the isochromosome of the short arm of chromosome 12 [i(12p)], has been 
      demonstrated in over 80 per cent of all histological varieties of testicular germ 
      cell tumours (TGCTs). In the remaining group of i(12p)-negative TGCTs, an 
      overrepresentation of chromosome 12p sequences has been found. The i(12p) 
      chromosome and overrepresentation of 12p sequences in CIS cells have also been 
      reported. In order to establish whether numerical and/or structural aberrations 
      of chromosome 12 can be found in CIS cells exfoliated into seminal fluid, semen 
      specimens from ten patients with CIS lesions were investigated using bicolour 
      double fluorescence in situ hybridization (FISH). The two DNA probes used, p 
      alpha 12H8 and YAC 5, specifically detect the centromeric region of chromosome 12 
      and a subregion, p11.2-p12.1, on the short arm of chromosome 12, respectively. 
      Ejaculates of ten azoospermic or oligozoospermic infertile males, presumably 
      CIS-free, were used as negative controls. Nuclei exhibiting three or more 
      chromosome 12 signals were found to be present in a significantly larger number 
      in the patient samples than in the control samples. Nuclei with five or more 
      chromosome 12 signals were observed in eight out of the ten patients. 
      Morphologically similar arrangements to i(12p) were observed in some of the 
      ejaculates. These results demonstrate the potential of FISH in the early 
      detection of CIS and TGCTs in males at high risk.
FAU - Meng, F J
AU  - Meng FJ
AD  - Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
FAU - Zhou, Y
AU  - Zhou Y
FAU - Giwercman, A
AU  - Giwercman A
FAU - Skakkebaek, N E
AU  - Skakkebaek NE
FAU - Geurts van Kessel, A D
AU  - Geurts van Kessel AD
FAU - Suijkerbuijk, R F
AU  - Suijkerbuijk RF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
SB  - IM
MH  - Adult
MH  - Carcinoma in Situ/diagnosis/*genetics
MH  - Case-Control Studies
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 12
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - *Isochromosomes
MH  - Lymphocytes/physiology
MH  - Male
MH  - Metaphase
MH  - Middle Aged
MH  - Semen/*cytology
MH  - Statistics, Nonparametric
MH  - Testicular Neoplasms/diagnosis/*genetics
EDAT- 1999/04/22 02:04
MHDA- 2000/06/20 09:00
CRDT- 1999/04/22 02:04
PHST- 1999/04/22 02:04 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/04/22 02:04 [entrez]
AID - 10.1002/(SICI)1096-9896(1998110)186:3<235::AID-PATH177>3.0.CO;2-U [pii]
AID - 10.1002/(SICI)1096-9896(1998110)186:3<235::AID-PATH177>3.0.CO;2-U [doi]
PST - ppublish
SO  - J Pathol. 1998 Nov;186(3):235-9. doi: 
      10.1002/(SICI)1096-9896(1998110)186:3<235::AID-PATH177>3.0.CO;2-U.