PMID- 10211125
OWN - NLM
STAT- MEDLINE
DCOM- 19990426
LR  - 20181201
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 186
IP  - 3
DP  - 1998 Nov
TI  - Detection of the bcr/abl gene in bone marrow macrophages in CML and alterations 
      during interferon therapy--a fluorescence in situ hybridization study on trephine 
      biopsies.
PG  - 331-5
AB  - A fluorescence in situ hybridization (FISH) study was performed on trephine 
      biopsies of the bone marrow in chronic myelogenous leukaemia (CML) to evaluate 
      the bcr/abl translocation in macrophages before and during interferon (IFN) 
      therapy. Mature macrophages and osteoclasts were identified by the monoclonal 
      antibody PG-M1, which recognizes a fixative-resistant epitope of the CD68 
      molecule. In contrast to a control group, in 145 of 479 (30 per cent) macrophages 
      of the CML bone marrow, a positive fusion signal was found, together with 
      corresponding translocation sites in the surrounding myeloid cells. In patients 
      following IFN treatment for approximately 12 months and with 
      haematological/cytogenetic remission by clinical standards, this number was 
      reduced to 10 of 136 (9 per cent) macrophages. In addition, the few 
      multinucleated osteoclasts of the untreated CML bone marrow displayed positive 
      translocation spots. This finding supports the hypothesis that stromal 
      macrophages and osteoclasts are of haematogenic stem cell origin. Moreover, 
      clinical remission and reduction of bcr/abl-positive macrophages under IFN 
      therapy lend support to the hypothesis that the presence of malignant stromal 
      macrophages may contribute to the selective expansion of leukaemic precursors and 
      the suppression of normal haematopoiesis in CML.
FAU - Thiele, J
AU  - Thiele J
AD  - Institute of Pathology, University of Cologne, Germany.
FAU - Schmitz, B
AU  - Schmitz B
FAU - Fuchs, R
AU  - Fuchs R
FAU - Kvasnicka, H M
AU  - Kvasnicka HM
FAU - Lorenzen, J
AU  - Lorenzen J
FAU - Fischer, R
AU  - Fischer R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Interferon alpha-2)
RN  - 0 (Interferon-alpha)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Bone Marrow Cells/*metabolism
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Interferon alpha-2
MH  - Interferon-alpha/*therapeutic use
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism/*pathology/therapy
MH  - Macrophages/immunology/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Osteoclasts/metabolism
MH  - Recombinant Proteins
EDAT- 1999/04/22 02:04
MHDA- 2000/06/20 09:00
CRDT- 1999/04/22 02:04
PHST- 1999/04/22 02:04 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/04/22 02:04 [entrez]
AID - 10.1002/(SICI)1096-9896(199811)186:3<331::AID-PATH178>3.0.CO;2-7 [pii]
AID - 10.1002/(SICI)1096-9896(199811)186:3<331::AID-PATH178>3.0.CO;2-7 [doi]
PST - ppublish
SO  - J Pathol. 1998 Nov;186(3):331-5. doi: 
      10.1002/(SICI)1096-9896(199811)186:3<331::AID-PATH178>3.0.CO;2-7.