PMID- 10211690
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20081121
IS  - 0002-9645 (Print)
IS  - 0002-9645 (Linking)
VI  - 60
IP  - 4
DP  - 1999 Apr
TI  - Evidence for divergence of restriction fragment length polymorphism patterns 
      following in vivo replication of porcine reproductive and respiratory syndrome 
      virus.
PG  - 463-7
AB  - OBJECTIVE: To determine stability of the restriction fragment length polymorphism 
      (RFLP) pattern of a porcine reproductive and respiratory syndrome vaccine virus 
      and patterns of other viral strains as they replicate in pigs. SAMPLE POPULATION: 
      Field samples of porcine reproductive and respiratory syndrome virus (PRRSV) and 
      samples from 2 weaned pigs, 2 nursery-age pigs, and 5 gilts experimentally 
      infected with PRRSV. PROCEDURE: PRRSV was isolated from field samples, 
      experimentally infected pigs, or pigs that were in contact with experimentally 
      infected pigs. For each virus, RNA was isolated from infected cells, and RFLP 
      patterns were determined. RESULTS: 61% of field samples had 2-5-2 RFLP patterns 
      characteristic of the vaccine virus, 32% had field virus RFLP patterns, and 7% 
      had intermediate RFLP patterns that indicated a virus with a close relationship 
      to the vaccine virus. Viruses isolated from experimentally infected pigs had no 
      change in RFLP patterns after up to 13 weeks of in vivo replication and 
      transmission to contact pigs. CONCLUSIONS AND CLINICAL RELEVANCE: RFLP patterns 
      distinguish the vaccine and field strains of PRRSV; however, as the vaccine virus 
      spreads among a swine population, the RFLP pattern can change to a related 
      intermediate pattern. A glycine at residue 151 of open reading frame 5 is another 
      marker for the vaccine virus; this glycine is rapidly lost and eventually 
      replaced with arginine as the vaccine virus replicates in pigs.
FAU - Wesley, R D
AU  - Wesley RD
AD  - Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA 50010, USA.
FAU - Mengeling, W L
AU  - Mengeling WL
FAU - Lager, K M
AU  - Lager KM
FAU - Vorwald, A C
AU  - Vorwald AC
FAU - Roof, M B
AU  - Roof MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Vet Res
JT  - American journal of veterinary research
JID - 0375011
SB  - IM
MH  - Animals
MH  - Female
MH  - *Genetic Variation
MH  - Male
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*physiology
MH  - Sequence Analysis/veterinary
MH  - Swine
MH  - *Virus Replication
EDAT- 1999/04/22 00:00
MHDA- 1999/04/22 00:01
CRDT- 1999/04/22 00:00
PHST- 1999/04/22 00:00 [pubmed]
PHST- 1999/04/22 00:01 [medline]
PHST- 1999/04/22 00:00 [entrez]
PST - ppublish
SO  - Am J Vet Res. 1999 Apr;60(4):463-7.