PMID- 10212401
OWN - NLM
STAT- MEDLINE
DCOM- 19990525
LR  - 20191103
IS  - 1354-2516 (Print)
IS  - 1354-2516 (Linking)
VI  - 3
IP  - 4
DP  - 1998 Mar
TI  - Characterization of calcium/calmodulin-dependent protein kinase II activity in 
      the nervous system of the lobster, Panulirus interruptus.
PG  - 335-45
AB  - Nervous system tissue from Panulirus interruptus has an enzyme activity that 
      behaves like calcium/calmodulin-dependent protein kinase II (CaM KII) This 
      activity phosphorylates known targets of CaM KII, such as synapsin I and 
      autocamtide 3. It is inhibited by a CaM KII-specific autoinhibitory domain 
      peptide. In addition, this lobster brain activity displays calcium-independent 
      activity after autophosphorylation, another characteristic of CaM KII. A cDNA 
      from the lobster nervous system was amplified using polymerase chain reaction. 
      The fragment was cloned and found to be structurally similar to CaM KII. Serum 
      from rabbits immunized with a fusion protein containing part of this sequence 
      immunoprecipitated a CaM KII enzyme activity and a family of phosphoproteins of 
      the appropriate size for CaM KII subunits. Lobster CaM KII activity is found in 
      the brain and stomatogastric nervous system including the commissural ganglia, 
      commissures, stomatogastric ganglion and stomatogastric nerve. Immunoblot 
      analysis of these same regions also identifies bands at an apparent molecular 
      weight characteristic of CaM KII.
FAU - Withers, M D
AU  - Withers MD
AD  - Volen Center, Brandeis University, Waltham, MA 02254, USA. 
      mwithers@volen.brandeis.edu
FAU - Kennedy, M B
AU  - Kennedy MB
FAU - Marder, E
AU  - Marder E
FAU - Griffith, L C
AU  - Griffith LC
LA  - eng
GR  - R01 GM054408/GM/NIGMS NIH HHS/United States
GR  - GM54408/GM/NIGMS NIH HHS/United States
GR  - NS17660/NS/NINDS NIH HHS/United States
GR  - NS17813/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Invert Neurosci
JT  - Invertebrate neuroscience : IN
JID - 9602489
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*analysis/genetics
MH  - Gene Expression Regulation, Enzymologic/physiology
MH  - Molecular Sequence Data
MH  - Nephropidae/*enzymology
MH  - Nervous System/*enzymology
MH  - Phosphorylation
MH  - Polymerase Chain Reaction
MH  - Precipitin Tests
MH  - Species Specificity
MH  - Stomach/innervation
EDAT- 1999/04/23 00:00
MHDA- 1999/04/23 00:01
CRDT- 1999/04/23 00:00
PHST- 1999/04/23 00:00 [pubmed]
PHST- 1999/04/23 00:01 [medline]
PHST- 1999/04/23 00:00 [entrez]
AID - 10.1007/BF02577693 [doi]
PST - ppublish
SO  - Invert Neurosci. 1998 Mar;3(4):335-45. doi: 10.1007/BF02577693.