PMID- 10213158 OWN - NLM STAT- MEDLINE DCOM- 19990610 LR - 20190701 IS - 0304-3940 (Print) IS - 0304-3940 (Linking) VI - 263 IP - 2-3 DP - 1999 Mar 26 TI - Brain-derived and glial cell line-derived neurotrophic factors protect a catecholaminergic cell line from dopamine-induced cell death. PG - 153-6 AB - Brain-derived neurotrophic factor (BDNF) promotes the survival of dopaminergic neurons in primary cultures and protects these neurons from the neurotoxic effects of 6-hydroxydopamine. The protective mechanism of BDNF on neurotoxicity was evaluated using CATH.a cells, a clonal catecholaminergic cell line derived from the central nervous system. Dopamine produced a dose-dependent cell death in CATH.a cells. Treatment of CATH.a cells with BDNF or glia cell line-derived neurotrophic factor (GDNF) reduced dopamine-induced cell death by approximately 60-70%. Nerve growth factor, basic fibroblast growth factor, neurotrophin-4/5 and insulin had no protective effect on dopamine-induced cell death. Dopamine decreased the activity of superoxide dismutase and the levels of glutathione in the CATH.a cells and these decreases were reversed by BDNF. In addition, BDNF treatment alone increased superoxide dismutase activity by 108%. These results suggest that BDNF may safeguard CATH.a cells from dopamine-induced cell death by maintaining or enhancing components of the cell, which protect from oxidative stress. FAU - Gong, L AU - Gong L AD - National Institute of Mental Health, Neuropsychiatry Branch, Bethesda, MD 20892-2668, USA. FAU - Wyatt, R J AU - Wyatt RJ FAU - Baker, I AU - Baker I FAU - Masserano, J M AU - Masserano JM LA - eng PT - Journal Article PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor) RN - 0 (Insulin) RN - 0 (Nerve Growth Factors) RN - 0 (Nerve Tissue Proteins) RN - 0 (Neuroprotective Agents) RN - 103107-01-3 (Fibroblast Growth Factor 2) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - GAN16C9B8O (Glutathione) RN - P658DCA9XD (neurotrophin 4) RN - VTD58H1Z2X (Dopamine) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*pharmacology/physiology MH - Cell Death/*drug effects MH - Cell Line MH - Central Nervous System MH - Dopamine/*toxicity MH - Fibroblast Growth Factor 2/pharmacology MH - Glial Cell Line-Derived Neurotrophic Factor MH - Glutathione/metabolism MH - Insulin/pharmacology MH - Kinetics MH - Nerve Growth Factors/pharmacology MH - Nerve Tissue Proteins/*pharmacology/physiology MH - Neuroprotective Agents/*pharmacology MH - Superoxide Dismutase/metabolism EDAT- 1999/04/23 00:00 MHDA- 1999/04/23 00:01 CRDT- 1999/04/23 00:00 PHST- 1999/04/23 00:00 [pubmed] PHST- 1999/04/23 00:01 [medline] PHST- 1999/04/23 00:00 [entrez] AID - S0304-3940(99)00148-2 [pii] AID - 10.1016/s0304-3940(99)00148-2 [doi] PST - ppublish SO - Neurosci Lett. 1999 Mar 26;263(2-3):153-6. doi: 10.1016/s0304-3940(99)00148-2.