PMID- 10213469
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20151119
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 56
IP  - 1
DP  - 1999 Apr 1
TI  - Expression of neurotrophins in the adult spinal cord in vivo.
PG  - 1-7
AB  - Potential roles of trophins in the normal and injured spinal cord are largely 
      undefined. However, a number of recent studies suggest that adult spinal cord 
      expresses neurotrophin receptors and responds to the neurotrophins, brain-derived 
      neurotrophic factor (BDNF) and neurotrophin 3 (NT3), particularly after injury. 
      The data indicate that trophins may enhance regrowth after damage and may 
      represent a new therapeutic approach to injury. Neurotrophins are reportedly 
      present in the spinal cord, but the cellular localization is unknown. This 
      information is critical to begin delineating mechanisms of actions. To approach 
      this problem, we examined whether spinal cord glia express BDNF and NT3 in vivo 
      and have begun to define cellular distribution. Specific antibodies directed 
      against the neurotrophins were utilized to visualize neurotrophin protein. 
      Initial studies indicated that small cells in the white matter of adult rat 
      spinal cord express BDNF and NT3. Large neurotrophin-positive neurons were also 
      identified in the ventral cord. To identify the neurotrophin-positive cells, 
      co-localization studies were performed utilizing neurotrophin polyclonal antisera 
      together with monoclonal antibodies directed against the astrocyte marker, glial 
      fibrillary acidic protein (GFAP). In the white matter of adult spinal cord, 
      GFAP-positive and GFAP-negative cells expressed BDNF and NT3. Our study suggests 
      that astrocyte and non-astrocyte cells provide trophic support to the adult 
      spinal cord.
FAU - Dreyfus, C F
AU  - Dreyfus CF
AD  - Department of Neuroscience and Cell Biology, UMDNJ/Robert Wood Johnson Medical 
      School, Piscataway, New Jersey 08854, USA.
FAU - Dai, X
AU  - Dai X
FAU - Lercher, L D
AU  - Lercher LD
FAU - Racey, B R
AU  - Racey BR
FAU - Friedman, W J
AU  - Friedman WJ
FAU - Black, I B
AU  - Black IB
LA  - eng
GR  - HD23315/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Biomarkers
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - Immunohistochemistry
MH  - Nerve Growth Factors/*genetics/metabolism
MH  - Neuroglia/*metabolism
MH  - Neurons/classification/cytology/*metabolism
MH  - Neurotrophin 3
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/*metabolism
MH  - Transcription, Genetic
EDAT- 1999/04/23 02:03
MHDA- 2000/06/20 09:00
CRDT- 1999/04/23 02:03
PHST- 1999/04/23 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/04/23 02:03 [entrez]
AID - 10.1002/(SICI)1097-4547(19990401)56:1<1::AID-JNR1>3.0.CO;2-3 [pii]
AID - 10.1002/(SICI)1097-4547(19990401)56:1<1::AID-JNR1>3.0.CO;2-3 [doi]
PST - ppublish
SO  - J Neurosci Res. 1999 Apr 1;56(1):1-7. doi: 
      10.1002/(SICI)1097-4547(19990401)56:1<1::AID-JNR1>3.0.CO;2-3.