PMID- 10213474
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20181201
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 56
IP  - 1
DP  - 1999 Apr 1
TI  - Differential mRNA expression and subcellular locations of PI3-kinase isoforms in 
      sympathetic and sensory neurons.
PG  - 44-53
AB  - Phosphatidylinositol 3-kinase (PI3-kinase) enzymes are key signalling molecules 
      in the PC12 and neuronal cell survival pathway and are also involved in the 
      regulation of retrograde axonal transport of nerve growth factor (NGF), with 
      sympathetic neurons more sensitive to the effects of wortmannin/LY294002 than 
      sensory neurons (Bartlett et al. [1997]; Brain Res. 761:257-262; Reynolds et al. 
      [1998] Brain Res. 798:67-74). In this article, we characterized the mRNA 
      expression of PI3-kinase isoforms in mouse sympathetic superior cervical ganglia 
      (SCG) and sensory trigeminal ganglia (TGG) and examined the subcellular locations 
      of immunoreactivity of the PI3-kinase isoforms in mouse cultured SCG and dorsal 
      root ganglion (DRG) neurons. Both the SCG and the TGG express mRNA for the 
      p110alpha, beta, gamma, delta, and vps34p PI3-kinase isoforms, but the TGG and 
      not the SCG express mRNA for the p170 PI3-kinase isoform. In cultured SCG and DRG 
      neurons, p110alpha, beta, and gamma immunoreactivity is in the SCG and DRG growth 
      cones, and predominantly in puncta throughout the growth cone varicosity. 
      However, in the cell bodies immunoreactivity varied, p110alpha is localized 
      predominantly at the plasma membrane, while p110beta and gamma is localized in 
      the perinuclear region of the cells. In addition, unlike other cell types, 
      wortmannin has little effect on actin filament polymerization in either mouse 
      cultured SCG or DRG neurons.
FAU - Bartlett, S E
AU  - Bartlett SE
AD  - Developmental Neurobiology, Division of Neuroscience, The John Curtin School of 
      Medical Research, Australian National University, Canberra, ACT. 
      selena.bartlett@anu.edu.au
FAU - Reynolds, A J
AU  - Reynolds AJ
FAU - Tan, T
AU  - Tan T
FAU - Heydon, K
AU  - Heydon K
FAU - Hendry, I A
AU  - Hendry IA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Actins)
RN  - 0 (Androstadienes)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 22144-77-0 (Cytochalasin D)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Actins/metabolism
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Base Sequence
MH  - Cell Membrane/enzymology
MH  - Cells, Cultured
MH  - Cerebellum/enzymology
MH  - Cytochalasin D/pharmacology
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Isoenzymes/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Neurons/*enzymology
MH  - Neurons, Afferent/*enzymology
MH  - Phosphatidylinositol 3-Kinases/*genetics/metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Spleen/enzymology
MH  - Subcellular Fractions/enzymology
MH  - Superior Cervical Ganglion/enzymology
MH  - *Transcription, Genetic/drug effects
MH  - Trigeminal Ganglion/enzymology
MH  - Wortmannin
EDAT- 1999/04/23 02:03
MHDA- 2000/06/20 09:00
CRDT- 1999/04/23 02:03
PHST- 1999/04/23 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/04/23 02:03 [entrez]
AID - 10.1002/(SICI)1097-4547(19990401)56:1<44::AID-JNR6>3.0.CO;2-6 [pii]
AID - 10.1002/(SICI)1097-4547(19990401)56:1<44::AID-JNR6>3.0.CO;2-6 [doi]
PST - ppublish
SO  - J Neurosci Res. 1999 Apr 1;56(1):44-53. doi: 
      10.1002/(SICI)1097-4547(19990401)56:1<44::AID-JNR6>3.0.CO;2-6.