PMID- 10214353
OWN - NLM
STAT- MEDLINE
DCOM- 19990507
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 110
IP  - 2
DP  - 1999 Apr 15
TI  - Prognostic relevance of monosomy at the 13q14 locus detected by fluorescence in 
      situ hybridization in B-cell chronic lymphocytic leukemia.
PG  - 77-81
AB  - Deletion of the chromosome band 13q14, which contains the putative deleted in 
      B-cell malignancy (DBM) gene, and trisomy 12 have been demonstrated by 
      fluorescence in situ hybridization (FISH) techniques in malignant B-cells from 
      patients with B cell chronic lymphocytic leukemia (B-CLL). However, the 
      prognostic relevance of 13q14 abnormalities as detected by FISH is unknown. We 
      prospectively studied malignant blood cells from 54 consecutive, untreated B-CLL 
      patients using FISH probes to the RB1 locus and DBM (markers D13S25 and D13S319) 
      for band 13q14, as well as probes to chromosome 12. The cells from all cases were 
      CD5+ CD20+, expressed clonally restricted surface immunoglobulin light chain, and 
      had typical features for B-CLL on careful blood smear morphologic evaluation. 
      Patients were followed for a mean of 3.9 years and treatment-free survival (TFS) 
      was used in the prognostic factor analysis. Twenty-four (44%) patients were 
      observed to have monosomy of the RB1 locus and 26 (48%) monosomy of D13S25 and 
      D13S319. The 26 patients who had a deletion at at least one of these loci had a 
      48.4 month (mo) median TFS vs 31.1 mo for those without evidence of deletion at 
      any 13q14 locus (p = 0.07). The seven patients found to have trisomy 12 had a 
      median TFS of 6.9 mo vs 39.3 mo for those diploid for chromosome 12 (p < 0.01). 
      When these seven patients with trisomy 12 were excluded from the analysis, 
      patients who had a deletion at 13q14 tended to have a longer median TFS (50.1 vs 
      36.2 mos), but this was not statistically significant (p = 0.2). This study 
      confirms the prevalence of 13q14 deletions in B-CLL and suggests that patients 
      with this abnormality have a better TFS than those with trisomy 12.
FAU - Hogan, W J
AU  - Hogan WJ
AD  - Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA.
FAU - Tefferi, A
AU  - Tefferi A
FAU - Borell, T J
AU  - Borell TJ
FAU - Jenkins, R
AU  - Jenkins R
FAU - Li, C Y
AU  - Li CY
FAU - Witzig, T E
AU  - Witzig TE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 13
MH  - Diploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/mortality
MH  - Male
MH  - Middle Aged
MH  - Monosomy/*genetics
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Survival Rate
EDAT- 1999/04/24 00:00
MHDA- 1999/04/24 00:01
CRDT- 1999/04/24 00:00
PHST- 1999/04/24 00:00 [pubmed]
PHST- 1999/04/24 00:01 [medline]
PHST- 1999/04/24 00:00 [entrez]
AID - S0165460898002076 [pii]
AID - 10.1016/s0165-4608(98)00207-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Apr 15;110(2):77-81. doi: 
      10.1016/s0165-4608(98)00207-6.