PMID- 10216147
OWN - NLM
STAT- MEDLINE
DCOM- 19990519
LR  - 20061115
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 29
IP  - 5
DP  - 1999 May
TI  - Permissiveness of human biliary epithelial cells to infection by hepatitis C 
      virus.
PG  - 1587-95
AB  - The cellular tropism of hepatitis C virus (HCV) is an important but much debated 
      issue. Permissivity to HCV of biliary cells has never been demonstrated. In this 
      context, we used gallbladder epithelial cells (GBEC) as a model of the more 
      proximal biliary epithelium. These cells were isolated from HCV-positive and 
      -negative individuals and cultured for up to 40 days. Biliary cells from 
      HCV-negative subjects were infected in vitro with various inocula. The retention 
      of GBEC functional characteristics was assessed by the expression of cystic 
      fibrosis transmembrane conductance regulator (CFTR). All 12 GBEC tested from 
      HCV-negative patients were successfully infected by HCV. This was assessed by: 1) 
      the detection of HCV-RNA positive and negative strands; 2) the detection of the 
      viral capsid by immunofluorescence; and 3) the combination of single-strand 
      conformation polymorphism (SSCP) and HVR1 sequence analysis demonstrating the 
      distinct majoritary HCV genomes in serum and in GBEC. The level of HCV RNA in 
      cell extracts and supernatants was low, but HCV infection was highly 
      reproducible. Our results expand those showing the cellular tropism of HCV, and 
      demonstrate the sensitivity of biliary cells to HCV infection. This might have an 
      important impact in terms of pathogenesis and pathological features of HCV 
      infection. In addition, given the easy access to these cells and the high 
      reproducibility of in vitro infection, they should constitute an important tool 
      for studies aimed at analyzing the issue of HCV penetration and neutralizing 
      antibodies.
FAU - Loriot, M A
AU  - Loriot MA
AD  - INSERM U370, Faculte de Medecine Necker, Paris, France.
FAU - Bronowicki, J P
AU  - Bronowicki JP
FAU - Lagorce, D
AU  - Lagorce D
FAU - Lakehal, F
AU  - Lakehal F
FAU - Persico, T
AU  - Persico T
FAU - Barba, G
AU  - Barba G
FAU - Mergey, M
AU  - Mergey M
FAU - Vons, C
AU  - Vons C
FAU - Franco, D
AU  - Franco D
FAU - Belghiti, J
AU  - Belghiti J
FAU - Giacca, M
AU  - Giacca M
FAU - Housset, C
AU  - Housset C
FAU - Brechot, C
AU  - Brechot C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (Cell Extracts)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Base Sequence/genetics
MH  - Biliary Tract/pathology/physiopathology/*virology
MH  - Cell Extracts/chemistry
MH  - Cells, Cultured
MH  - Disease Susceptibility
MH  - Epithelial Cells/physiology/virology
MH  - Hepacivirus/genetics
MH  - *Hepatitis C, Chronic/pathology/virology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phenotype
MH  - RNA, Viral/analysis
EDAT- 1999/04/27 00:00
MHDA- 1999/04/27 00:01
CRDT- 1999/04/27 00:00
PHST- 1999/04/27 00:00 [pubmed]
PHST- 1999/04/27 00:01 [medline]
PHST- 1999/04/27 00:00 [entrez]
AID - S0270913999002414 [pii]
AID - 10.1002/hep.510290527 [doi]
PST - ppublish
SO  - Hepatology. 1999 May;29(5):1587-95. doi: 10.1002/hep.510290527.