PMID- 10216212
OWN - NLM
STAT- MEDLINE
DCOM- 19990526
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 425
IP  - 2
DP  - 1999 Apr 6
TI  - Co-localization of mitochondrial and double minute DNA in the nuclei of HL-60 
      cells but not normal cells.
PG  - 195-204
AB  - In an attempt to isolate genes located on double minute (Dmin) DNA in HL-60 
      cells, we prepared DNA probe from purified micronuclei. Micronucleation was 
      induced in HL-60 cells by treatment with hydroxyurea. Screening of a cDNA library 
      unexpectedly produced a number of clones containing mitochondrial DNA (mtDNA) 
      sequences. Here, we show that amplified mtDNA sequences were localized in nuclei 
      and micronuclei of HL-60 and COLO 320DM cells, but not in nuclei of WI-38 normal 
      human fibroblasts or peripheral blood T-cells. To unequivocally demonstrate the 
      presence of mtDNA inside of nuclei and micronuclei, we obtained tomographic 
      fluorescence in situ hybridization (FISH) images of mtDNA by confocal microscopy 
      of consecutive sections of paraformaldehyde (PFA)-fixed material. We also located 
      mtDNA in nuclear buds and purified micronuclei. Dmin DNA and mtDNA were always 
      located at similar sites. The mechanisms of nuclear retention of mtDNA and Dmin 
      DNA and the resulting influence on tumorigenesis are discussed.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Hirano, T
AU  - Hirano T
AD  - Life Science Group, Faculty of Integrated Arts and Sciences, Hiroshima 
      University, Kagamiyama 1-7-1, Higashihiroshima 739-8521, Japan.
FAU - Shiraishi, K
AU  - Shiraishi K
FAU - Adachi, K
AU  - Adachi K
FAU - Miura, S
AU  - Miura S
FAU - Watanabe, H
AU  - Watanabe H
FAU - Utiyama, H
AU  - Utiyama H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (DNA, Neoplasm)
RN  - X6Q56QN5QC (Hydroxyurea)
SB  - IM
MH  - Cell Cycle/drug effects
MH  - Cell Nucleus/*chemistry
MH  - Cell Transformation, Neoplastic/genetics
MH  - Colonic Neoplasms/pathology
MH  - *DNA Damage
MH  - DNA, Mitochondrial/*analysis
MH  - DNA, Neoplasm/*analysis
MH  - Fibroblasts/chemistry/ultrastructure
MH  - Gene Amplification
MH  - HL-60 Cells/chemistry/drug effects/*ultrastructure
MH  - Humans
MH  - Hydroxyurea/pharmacology
MH  - In Situ Hybridization, Fluorescence
MH  - Micronuclei, Chromosome-Defective/chemistry/drug effects/*ultrastructure
MH  - Microscopy, Confocal
MH  - T-Lymphocytes/chemistry/ultrastructure
MH  - Tumor Cells, Cultured/chemistry/ultrastructure
EDAT- 1999/04/27 00:00
MHDA- 1999/04/27 00:01
CRDT- 1999/04/27 00:00
PHST- 1999/04/27 00:00 [pubmed]
PHST- 1999/04/27 00:01 [medline]
PHST- 1999/04/27 00:00 [entrez]
AID - S0027-5107(99)00037-8 [pii]
AID - 10.1016/s0027-5107(99)00037-8 [doi]
PST - ppublish
SO  - Mutat Res. 1999 Apr 6;425(2):195-204. doi: 10.1016/s0027-5107(99)00037-8.