PMID- 10216255
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20191210
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 230
IP  - 2
DP  - 1999 Apr 16
TI  - Gene structure and chromosomal localization of mouse cyclin G2 (Ccng2).
PG  - 171-80
AB  - Cyclins are essential activators of cyclin-dependent kinases (Cdk) which, in 
      turn, play pivotal roles in controlling transition through cell-cycle 
      checkpoints. Cyclin G2 is a recently discovered second member of the G-type 
      cyclins. The two members of the G-type cyclins, cyclin G1 and cyclin G2, share 
      high structural similarity but their function remains to be defined. Here we 
      characterize the structure of the mouse cyclin G2 gene by first cloning and 
      sequencing the full-length mouse cyclin G2 cDNA. The cyclin G2 cDNA was used to 
      isolate the cyclin G2 gene from a BAC library and to establish that the gene was 
      transcribed from eight exons spanning a total of 8604bp. The cyclin G2 gene was 
      mapped by fluorescence in situ hybridization (FISH) to mouse chromosome 
      5E3.3.-F1.3. This region is syntenic to a region on human chromosome 4. The 
      expression of cyclins G1 and G2 was examined in various tissues, but no 
      correlation between expression patterns of the two genes was observed. However, 
      during hepatic ontogenesis the cyclin G2 expression level decreased with age, 
      whereas cyclin G1 expression increased. Transient expression of cyclin G2-green 
      fluorescent protein (GFP) fusion protein in NIH3T3 cells showed that cyclin G2 is 
      essentially a cytoplasmic protein, in contrast to the largely nuclear 
      localization of cyclin G1. Our data suggest that, despite the close structural 
      similarity between mouse cyclins G1 and G2, these proteins most likely perform 
      distinct functions.
FAU - Jensen, M R
AU  - Jensen MR
AD  - Laboratory of Experimental Carcinogenesis, Division of Basic Sciences, National 
      Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Audolfsson, T
AU  - Audolfsson T
FAU - Keck, C L
AU  - Keck CL
FAU - Zimonjic, D B
AU  - Zimonjic DB
FAU - Thorgeirsson, S S
AU  - Thorgeirsson SS
LA  - eng
SI  - GENBANK/AF005885
SI  - GENBANK/AF079877
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (CCNG2 protein, human)
RN  - 0 (Ccng1 protein, mouse)
RN  - 0 (Cyclin G1)
RN  - 0 (Cyclin G2)
RN  - 0 (Cyclins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Luminescent Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - 3T3 Cells
MH  - Aging/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 5
MH  - Cloning, Molecular
MH  - Cyclin G1
MH  - Cyclin G2
MH  - Cyclins/chemistry/*genetics
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Transfection
EDAT- 1999/04/27 00:00
MHDA- 1999/04/27 00:01
CRDT- 1999/04/27 00:00
PHST- 1999/04/27 00:00 [pubmed]
PHST- 1999/04/27 00:01 [medline]
PHST- 1999/04/27 00:00 [entrez]
AID - S0378111999000578 [pii]
AID - 10.1016/s0378-1119(99)00057-8 [doi]
PST - ppublish
SO  - Gene. 1999 Apr 16;230(2):171-80. doi: 10.1016/s0378-1119(99)00057-8.