PMID- 10216916
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20191103
IS  - 0967-1994 (Print)
IS  - 0967-1994 (Linking)
VI  - 7
IP  - 1
DP  - 1999 Feb
TI  - Glucose metabolism during the final stage of human oocyte maturation: genetic 
      expression of hexokinase, glucose phosphate isomerase and phosphofructokinase.
PG  - 45-50
AB  - The low involvement of glucose metabolism in early preimplantation embryos has 
      suggested the presence of metabolic blocks in the glycolytic pathway. Genetic 
      expression of hexokinase (HK), glucose-6-phosphate isomerase (GPI) or 
      phosphofructokinase (PFK) were qualitatively analysed using the reverse 
      transcription nested polymerase chain reaction (RT-nested PCR) in individual 
      human immature oocytes at the germinal vesicle (GV) stage and in single 
      non-fertilised human metaphase II (MII) oocytes, after IVF or ICSI failures. 
      Transcripts encoding for HK, GPI and PFK were detectable in the majority of the 
      oocytes analysed, and with different expression patterns. GPI transcripts were 
      consistently present and appear to be constitutively expressed during GV and MII 
      stages. In contrast, low quantities of transcripts encoding for HK and PFK were 
      observed in all oocytes analysed. Our data revealed that the metabolic block 
      previously reported at GPI or PFK levels underwent post-transcriptional 
      regulation. The expression profiles of HK and PFK transcripts in GV and MII 
      reflect a low level of transcription or active translation of maternal 
      transcripts during oocyte maturation. Nevertheless, enzymatic activities of HK 
      and PFK have previously been determined in human oocytes. This suggests that HK 
      and PFK may be accumulated in protein (enzyme) form instead of maternal mRNA 
      during human oocyte maturation. The expression pathway of glycolytic metabolism 
      reflects the presence of different mechanisms involved in gene 
      expression/regulation at the transcriptional and translational level and their 
      accumulation during human oocyte maturation.
FAU - el Mouatassim, S
AU  - el Mouatassim S
AD  - Laboratoire Marcel Merieux, Lyon, France.
FAU - Hazout, A
AU  - Hazout A
FAU - Bellec, V
AU  - Bellec V
FAU - Menezo, Y
AU  - Menezo Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Zygote
JT  - Zygote (Cambridge, England)
JID - 9309124
RN  - 0 (DNA Primers)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - DNA Primers
MH  - Female
MH  - *Gene Expression Regulation, Enzymologic
MH  - Glucose/*metabolism
MH  - Glucose-6-Phosphate Isomerase/*genetics
MH  - Hexokinase/*genetics
MH  - Humans
MH  - Metaphase
MH  - Oocytes/cytology/*physiology
MH  - Phosphofructokinase-1/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription, Genetic
EDAT- 1999/04/27 00:00
MHDA- 1999/04/27 00:01
CRDT- 1999/04/27 00:00
PHST- 1999/04/27 00:00 [pubmed]
PHST- 1999/04/27 00:01 [medline]
PHST- 1999/04/27 00:00 [entrez]
AID - 10.1017/s0967199499000386 [doi]
PST - ppublish
SO  - Zygote. 1999 Feb;7(1):45-50. doi: 10.1017/s0967199499000386.