PMID- 10217072
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20191024
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 33
IP  - 2
DP  - 1999
TI  - Flow cytometric analysis of genetic damage, effect on cell cycle progression, and 
      apoptosis by thiophanate-methyl in human lymphocytes.
PG  - 173-6
AB  - Flow cytometric technique was used to study the effects of the fungicide 
      Thiophanate-methyl on cell proliferation, micronucleus induction, and apoptosis 
      in human peripheral blood lymphocytes treated in vitro. In particular, a combined 
      approach of flow cytometry and fluorescence in situ hybridization (FISH) with a 
      pancentromeric alpha-satellite probe was used to evaluate the mechanism of 
      micronucleus induction by Thiophanate-methyl. Flow sorted micronuclei (MN) 
      induced in human lymphocytes by Thiophanate-methyl were analyzed by FISH and the 
      results were compared with results from FISH analysis on MN in binucleated cells. 
      It could be shown that most MN induced by Thiophanate-methyl did not reveal any 
      centromeric signal, thus demonstrating clastogenic action of this fungicide. 
      Moreover, it was found that as a function of the concentration of 
      Thiophanate-methyl, cellular proliferation was delayed and the frequency of 
      apoptotic cells was increased.
FAU - Fimognari, C
AU  - Fimognari C
AD  - Department of Pharmacology, University of Bologna, Italy.
FAU - Nusse, M
AU  - Nusse M
FAU - Hrelia, P
AU  - Hrelia P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 5Q0Y96D5I8 (Thiophanate)
SB  - IM
MH  - Adult
MH  - Apoptosis/*drug effects
MH  - Cell Cycle/*drug effects
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Lymphocytes/cytology/*drug effects
MH  - Male
MH  - Thiophanate/*toxicity
EDAT- 1999/04/27 00:00
MHDA- 1999/04/27 00:01
CRDT- 1999/04/27 00:00
PHST- 1999/04/27 00:00 [pubmed]
PHST- 1999/04/27 00:01 [medline]
PHST- 1999/04/27 00:00 [entrez]
AID - 10.1002/(SICI)1098-2280(1999)33:2<173::AID-EM10>3.0.CO;2-R [pii]
AID - 10.1002/(sici)1098-2280(1999)33:2<173::aid-em10>3.0.co;2-r [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 1999;33(2):173-6. doi: 
      10.1002/(sici)1098-2280(1999)33:2<173::aid-em10>3.0.co;2-r.