PMID- 10218870
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20191210
IS  - 0028-3908 (Print)
IS  - 0028-3908 (Linking)
VI  - 38
IP  - 2
DP  - 1999 Feb
TI  - BDNF and NT-3 but not CNTF counteract the Ca2+ ionophore-induced apoptosis of 
      cultured cortical neurons: involvement of dual pathways.
PG  - 283-8
AB  - The effect of neurotrophic factors on apoptosis induced by ionomycin, a potent 
      Ca2+ ionophore, was investigated using cultured cortical neurons from embryonic 
      rats. Brain-derived neurotophic factor (BDNF) and neurotrophin-3 (NT-3) prevented 
      the ionomycin-mediated cell death in a dose-dependent manner. In contrast to the 
      neurotrophins, cilliary neurotrophic factor (CNTF) did not rescue neurons from 
      cell death induced by ionomycin. The protective effect of BDNF was partially 
      blocked by wortmannin, a phosphatidylinositol 3-kinase inhibitor, and by PD98059, 
      a MAP kinase kinase inhibitor. However, the addition of both compounds together 
      completely inhibited the survival promoting effect of BDNF. These results suggest 
      that the neuroprotective effect of BDNF requires activation of both 
      phosphatidylinositol-3 kinase and the Ras/MAP kinase cascade and that CNTF 
      signaling through other pathways is without an effect in this system.
FAU - Takei, N
AU  - Takei N
AD  - Department of Applied Biology, Kyoto Institute of Technology, Sakyo, Japan. 
      nobtak@bri.niigata-u.ac.jp
FAU - Tanaka, O
AU  - Tanaka O
FAU - Endo, Y
AU  - Endo Y
FAU - Lindholm, D
AU  - Lindholm D
FAU - Hatanaka, H
AU  - Hatanaka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Androstadienes)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 56092-81-0 (Ionomycin)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Apoptosis/drug effects/*physiology
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology
MH  - Ciliary Neurotrophic Factor
MH  - Dose-Response Relationship, Drug
MH  - Embryo, Mammalian
MH  - Enzyme Inhibitors/pharmacology
MH  - Flavonoids/pharmacology
MH  - Ionomycin/*pharmacology
MH  - Mitogen-Activated Protein Kinase Kinases
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neurons/*cytology/drug effects/*physiology
MH  - Neuroprotective Agents/pharmacology
MH  - Neurotrophin 3
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Protein Kinase Inhibitors
MH  - Rats
MH  - Rats, Wistar
MH  - Wortmannin
EDAT- 1999/04/28 00:00
MHDA- 1999/04/28 00:01
CRDT- 1999/04/28 00:00
PHST- 1999/04/28 00:00 [pubmed]
PHST- 1999/04/28 00:01 [medline]
PHST- 1999/04/28 00:00 [entrez]
AID - S0028390898001890 [pii]
AID - 10.1016/s0028-3908(98)00189-0 [doi]
PST - ppublish
SO  - Neuropharmacology. 1999 Feb;38(2):283-8. doi: 10.1016/s0028-3908(98)00189-0.