PMID- 10218903
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20210223
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 263
IP  - 1
DP  - 1999 Mar 19
TI  - Differential cholera-toxin sensitivity of supraspinal antinociception induced by 
      the cannabinoid agonists delta9-THC, WIN 55,212-2 and anandamide in mice.
PG  - 29-32
AB  - Intracerebroventricular (i.c.v.) administration to mice of 
      delta9-tetrahydrocannabinol (delta9-THC), WIN 55,212-2 or the endogenous 
      cannabinoid anandamide induced dose-related antinociception in the 55 degrees C 
      warm-water tail-flick test. Pretreatment (24 h, i.c.v.) with pertussis toxin 
      dose-dependently reduced the antinociceptive effect of delta9-THC (955 nmol), WIN 
      55,212-2 (30 nmol) and anandamide (135 nmol) (IC50 = 0.13, 5.5, and 0.32 nmol, 
      respectively). In contrast, pretreatment (24 h, i.c.v.) with cholera toxin 
      (0.1-3.0 mg) reduced the antinociception of WIN 55,212-2, had minimal effect on 
      delta9-THC, and dose-dependently increased the antinociception of anandamide 
      (ED50 = 0.50 nmol). These data suggest differences in the receptor-effector 
      coupling of delta9-THC, WIN 55,212-2 and anandamide in supraspinal-induced 
      antinociception in mice.
FAU - Raffa, R B
AU  - Raffa RB
AD  - Temple University School of Pharmacy, Philadelphia, PA 19140-5104, USA.
FAU - Stone, D J Jr
AU  - Stone DJ Jr
FAU - Hipp, S J
AU  - Hipp SJ
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Analgesics)
RN  - 0 (Arachidonic Acids)
RN  - 0 (Benzoxazines)
RN  - 0 (Cannabinoids)
RN  - 0 (Endocannabinoids)
RN  - 0 (Morpholines)
RN  - 0 (Naphthalenes)
RN  - 0 (Polyunsaturated Alkamides)
RN  - 5H31GI9502 
      ((3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanone)
RN  - 9012-63-9 (Cholera Toxin)
RN  - UR5G69TJKH (anandamide)
SB  - IM
MH  - Analgesics/administration & dosage/*pharmacology
MH  - Animals
MH  - Arachidonic Acids/administration & dosage/*pharmacology
MH  - Benzoxazines
MH  - Cannabinoids/administration & dosage/*pharmacology
MH  - Cerebral Ventricles/drug effects/*physiology
MH  - Cholera Toxin/administration & dosage/*pharmacology
MH  - Endocannabinoids
MH  - Injections, Intraventricular
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Morpholines/administration & dosage/*pharmacology
MH  - Naphthalenes/administration & dosage/*pharmacology
MH  - Pain/*physiopathology/prevention & control
MH  - Polyunsaturated Alkamides
MH  - Spinal Cord/drug effects/*physiology/physiopathology
EDAT- 1999/04/28 02:03
MHDA- 2001/03/28 10:01
CRDT- 1999/04/28 02:03
PHST- 1999/04/28 02:03 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/04/28 02:03 [entrez]
AID - S0304-3940(99)00096-8 [pii]
AID - 10.1016/s0304-3940(99)00096-8 [doi]
PST - ppublish
SO  - Neurosci Lett. 1999 Mar 19;263(1):29-32. doi: 10.1016/s0304-3940(99)00096-8.