PMID- 10219338
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190905
IS  - 0098-1532 (Print)
IS  - 0098-1532 (Linking)
VI  - 32
IP  - 5
DP  - 1999 May
TI  - Acquired X-chromosome aneuploidy in children with acute lymphoblastic leukemia.
PG  - 360-5
AB  - BACKGROUND: A cytogenetic study of 75 consecutive children with ALL revealed a 
      normal karyotype, a low hyperdiploid karyotype (including 47-50 chromosomes), and 
      a high hyperdiploid karyotype (including > 50 chromosomes) in 10, 12, and 33 
      patients, respectively. An acquired extra X-chromosome was detected at diagnosis 
      by conventional cytogenetics in 29 (88%) of 33 children with a high hyperdiploid 
      karyotype and in 4 (33%) of 12 children with a low hyperdiploid karyotype. 
      X-chromosome aneuploidy was retrospectively studied by fluorescence in situ 
      hybridization (FISH) in eight and 20 patients with a normal and a hyperdiploid 
      karyotype, respectively. PROCEDURE: A classical cytogenetic study was performed 
      according to standard methods. FISH with the centromeric probe specific to 
      X-chromosome was used to study interphase cells of bone marrow or blood samples. 
      RESULTS: An extra X-chromosome was found by FISH in all 13 patients with a high 
      hyperdiploid or tetraploid, in 6 of 7 patients with a low hyperdiploid, and in 
      none with a normal karyotype. Two children with a normal karyotype displayed 
      monosomy X. Altogether, 57.3% of newly diagnosed children displayed X-chromosome 
      aneuploidy. CONCLUSIONS: Out study indicates that X-chromosome aneuploidy may be 
      the most common chromosome abnormality in childhood ALL. It can be detected in 
      nearly all children with a high hyperdiploid karyotype and up to one-half of the 
      patients with a low hyperdiploid karyotype. FISH with an X-chromosome centromeric 
      probe is a rapid and simple tool to detect an abnormal clone at diagnosis in the 
      majority of children with ALL and is useful in confirming remission in these 
      patients.
FAU - Heinonen, K
AU  - Heinonen K
AD  - Chromosome and DNA Laboratory, Kuopio University Hospital, Finland. 
      kristiina.heinonen@kuh.fi
FAU - Mahlamaki, E
AU  - Mahlamaki E
FAU - Riikonen, P
AU  - Riikonen P
FAU - Meltoranta, R L
AU  - Meltoranta RL
FAU - Rahiala, J
AU  - Rahiala J
FAU - Perkkio, M
AU  - Perkkio M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Med Pediatr Oncol
JT  - Medical and pediatric oncology
JID - 7506654
SB  - IM
MH  - Adolescent
MH  - *Aneuploidy
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Incidence
MH  - Infant
MH  - Karyotyping
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Retrospective Studies
MH  - *X Chromosome
EDAT- 1999/04/29 02:03
MHDA- 2000/06/20 09:00
CRDT- 1999/04/29 02:03
PHST- 1999/04/29 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/04/29 02:03 [entrez]
AID - 10.1002/(SICI)1096-911X(199905)32:5<360::AID-MPO9>3.0.CO;2-7 [pii]
AID - 10.1002/(sici)1096-911x(199905)32:5<360::aid-mpo9>3.0.co;2-7 [doi]
PST - ppublish
SO  - Med Pediatr Oncol. 1999 May;32(5):360-5. doi: 
      10.1002/(sici)1096-911x(199905)32:5<360::aid-mpo9>3.0.co;2-7.