PMID- 10220169
OWN - NLM
STAT- MEDLINE
DCOM- 19990908
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 145 ( Pt 4)
DP  - 1999 Apr
TI  - Genotypic analysis of Mycobacterium tuberculosis from medieval human remains.
PG  - 899-904
LID - 10.1099/13500872-145-4-899 [doi]
AB  - Three medieval bone samples with osteological evidence of tuberculosis infection 
      were analysed for the presence of DNA sequences from Mycobacterium tuberculosis 
      using a series of PCRs. In each case amplification of IS6110 and part of the 
      beta-subunit of RNA polymerase identified infection with a bacterium belonging to 
      the M. tuberculosis complex. Amplification of the mtp40 genome fragment and the 
      presence of a guanine residue at position 285 in the oxyR pseudogene, 
      demonstrated the infecting strain to be similar to present day M. tuberculosis 
      isolates rather than to Mycobacterium bovis. Spoligotyping, based on 
      amplification of the direct repeat (DR) region of the mycobacterial genome, 
      provided further evidence of similarity to M. tuberculosis and indicated a close 
      relationship between isolates associated with two separate medieval burials. The 
      study demonstrates the feasibility of amplifying multiple M. tuberculosis loci in 
      ancient human remains and suggests important applications in the study of the 
      palaeoepidemiology and virulence of tuberculosis in past populations.
FAU - Taylor, G M
AU  - Taylor GM
AD  - Department of Infectious Diseases and Microbiology, Imperial College School of 
      Medicine, St Mary's Campus, Norfolk Place, London W2 1PG, UK.
FAU - Goyal, M
AU  - Goyal M
AD  - Department of Biosciences, University of Hertfordshire, Hatfield, Herts AL10 9AB, 
      UK.
FAU - Legge, A J
AU  - Legge AJ
AD  - Faculty of Continuing Education, Birkbeck College, 26 Russell Square, London WC1B 
      5DQ, UK.
FAU - Shaw, R J
AU  - Shaw RJ
AD  - Department of Respiratory Medicine, Imperial College School of Medicine, St 
      Mary's Campus, Norfolk Place, London W2 1PG, UK.
FAU - Young, D
AU  - Young D
AD  - Department of Infectious Diseases and Microbiology, Imperial College School of 
      Medicine, St Mary's Campus, Norfolk Place, London W2 1PG, UK.
LA  - eng
PT  - Historical Article
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oligonucleotides)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.7.6 (DNA-Directed RNA Polymerases)
RN  - EC 2.7.7.6 (RNA polymerase beta subunit)
SB  - IM
MH  - Bacterial Typing Techniques
MH  - Bone and Bones/*microbiology/pathology
MH  - DNA Transposable Elements
MH  - DNA, Bacterial/*analysis/isolation & purification
MH  - *DNA-Binding Proteins
MH  - DNA-Directed RNA Polymerases/genetics
MH  - Evolution, Molecular
MH  - History, Medieval
MH  - Humans
MH  - Male
MH  - Mycobacterium tuberculosis/classification/*genetics/isolation & purification
MH  - Oligonucleotides/analysis
MH  - Paleopathology
MH  - Pseudogenes
MH  - Repressor Proteins/genetics
MH  - Reproducibility of Results
MH  - Transcription Factors/genetics
MH  - Tuberculosis, Osteoarticular/*history/microbiology
EDAT- 1999/04/29 00:00
MHDA- 1999/04/29 00:01
CRDT- 1999/04/29 00:00
PHST- 1999/04/29 00:00 [pubmed]
PHST- 1999/04/29 00:01 [medline]
PHST- 1999/04/29 00:00 [entrez]
AID - 10.1099/13500872-145-4-899 [doi]
PST - ppublish
SO  - Microbiology (Reading). 1999 Apr;145 ( Pt 4):899-904. doi: 
      10.1099/13500872-145-4-899.