PMID- 10222217
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20220317
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 7
IP  - 2
DP  - 1999 Mar
TI  - The effects of hyaluronan on matrix metalloproteinase-3 (MMP-3), 
      interleukin-1beta(IL-1beta), and tissue inhibitor of metalloproteinase-1 (TIMP-1) 
      gene expression during the development of osteoarthritis.
PG  - 182-90
AB  - OBJECTIVE: To assess the influence of intra-articular injection of hyaluronan 
      (HA) on expression of matrix metalloproteinase-3 (MMP-3), 
      interleukin-1beta(IL-1beta), and tissue inhibitor of metalloproteinase-1 (TIMP-1) 
      in cartilage and synovium during the process of osteoarthritis (OA). DESIGN: 
      Eighteen mature New Zealand white rabbits underwent unilateral anterior cruciate 
      ligament transection (ACLT) and were divided into two groups. The first group (HA 
      injection group) received 0.3 ml of intra-articular HA injections into the ACLT 
      knees 4 weeks after transection, once a week for 5 weeks as per clinical 
      treatment presently utilized. The animals in the second group (no injection 
      group) were not injected after ACLT. At death, 9 weeks following surgery, 
      synovium and cartilage were harvested and total RNA was extracted. Gene 
      expressions of MMP-3, IL-1beta and TIMP-1 were analyzed using reverse 
      transcription-polymerase chain reaction (RT-PCR) for each subgroup created 
      according to morphological grade of OA. RESULTS: The extent and grade of 
      cartilage damage in the HA injection group was less severe than in the no 
      injection group. In synovium, expression of MMP-3 and IL-1beta mRNA was 
      suppressed in the mild grades of OA in the HA injection group. HA treatment had 
      either no effect on MMP-3 expression in cartilage at all grades of OA or on 
      enhanced MMP-3 and IL-1beta expression in synovium at a progressed grade. No 
      effect of HA treatment on TIMP-1 expression was observed in either cartilage or 
      synovium. CONCLUSIONS: These results suggest that one of the mechanism of 
      therapeutic effect of HA is down-regulation of MMP-3 and IL-1beta in synovium 
      during early development of OA.
CI  - Copyright 1999 OsteoArthritis Research Society International.
FAU - Takahashi, K
AU  - Takahashi K
AD  - Department of Orthopaedics, University of California San Diego-School of 
      Medicine, 9500 Gilman Dr., La Jolla, CA, 92093-0630, USA.
FAU - Goomer, R S
AU  - Goomer RS
FAU - Harwood, F
AU  - Harwood F
FAU - Kubo, T
AU  - Kubo T
FAU - Hirasawa, Y
AU  - Hirasawa Y
FAU - Amiel, D
AU  - Amiel D
LA  - eng
GR  - AG07996/AG/NIA NIH HHS/United States
GR  - AR07484/AR/NIAMS NIH HHS/United States
GR  - AR28467/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Anterior Cruciate Ligament/drug effects/metabolism
MH  - Down-Regulation/*drug effects
MH  - Hyaluronic Acid/*pharmacology
MH  - Interleukin-1/*biosynthesis/genetics
MH  - Matrix Metalloproteinase 3/biosynthesis/*drug effects/genetics
MH  - Osteoarthritis, Knee/*metabolism/pathology
MH  - RNA, Messenger/genetics
MH  - Rabbits
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synovial Membrane/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/biosynthesis/*drug effects/genetics
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - S1063-4584(98)90207-2 [pii]
AID - 10.1053/joca.1998.0207 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 1999 Mar;7(2):182-90. doi: 10.1053/joca.1998.0207.