PMID- 10222229
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 258
IP  - 1
DP  - 1999 Apr 29
TI  - Effect of the selection marker on the viability and plasmid stability of two 
      human proteins with neurotrophic action expressed in Escherichia coli.
PG  - 29-31
AB  - Most developed expression systems rely on the production of fusion proteins or 
      the change of selection marker increasing genetic stability to avoid toxicity of 
      heterologous proteins to Escherichia coli host cells. According to this, we 
      analyzed the effect of the selection marker on the viability and plasmid 
      stability of vectors pYMK5 and pYMK7 that codify neurotrophic factors 
      brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). We also 
      analyzed the influence of two different lac promoter inducers on these 
      parameters. We found that the addition of IPTG to culture medium produced a 
      significant decrease of viability and plasmid stability for both expression 
      vectors compared with values reached with lactose. There was no increase of both 
      parameters when we changed the selection marker, so we can conclude that, in our 
      case, a change of antibiotic does not solve the problem of low plasmid stability 
      values.
CI  - Copyright 1999 Academic Press.
FAU - Mari, Y M
AU  - Mari YM
AD  - Pharmaceutical Division, Biopharmaceutical Development Division, Center for 
      Genetic Engineering and Biotechnology, Playa 10600, Havana, Cuba.
FAU - Espinosa, A E
AU  - Espinosa AE
FAU - Ubieta, R
AU  - Ubieta R
FAU - Batista, O F
AU  - Batista OF
FAU - Fernandez, M L
AU  - Fernandez ML
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Genetic Markers)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cloning, Molecular
MH  - Escherichia coli/genetics
MH  - Genetic Markers
MH  - Humans
MH  - Lac Operon
MH  - Nerve Growth Factors/*genetics/metabolism
MH  - *Plasmids
MH  - Promoter Regions, Genetic
MH  - Recombinant Proteins/genetics/metabolism
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - S0006291X99905685 [pii]
AID - 10.1006/bbrc.1999.0568 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Apr 29;258(1):29-31. doi: 
      10.1006/bbrc.1999.0568.