PMID- 10222241
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 258
IP  - 1
DP  - 1999 Apr 29
TI  - Search for genes potentially involved in Mycobacterium tuberculosis virulence by 
      mRNA differential display.
PG  - 94-101
AB  - An mRNA differential display (DD) assay was developed to compare gene expression 
      between Mycobacterium tuberculosis H37Rv and its avirulent mutant H37Ra. The DD 
      protocol made use of an oligo(dT) to prime reverse-transcriptase (RT)-dependent 
      transcription of poly-A tailed mRNAs and a PCR amplification of the RT products 
      by using ten 12-base arbitrary primers in all their pair combinations. This 
      analysis yielded 745 and 708 bands, including 52 and 15 differentially generated 
      bands, in the strains H37Rv and H37Ra, respectively. Six cDNAs that appeared to 
      be expressed in H37Rv, but not in H37Ra, were reamplified and cloned and at least 
      10 inserts were sequenced for each cloned cDNA. After resolving discrepant 
      results, 6 inserts were found highly homologous to M. tuberculosis H37Rv genes. 
      Three of these, i.e., genes Rv2770c, Rv1345, and Rv0288, coding respectively for 
      a member of the PPE protein family, a probable polyketide synthase, and a member 
      of the protein family containing ESAT-6, have been predictively associated to 
      immunological or pathogenetic aspects of M. tuberculosis infection; the other 
      genes, i.e., Rv2336, Rv1320c, and Rv2819c, code for proteins with unknown 
      functions. These results show that mRNA DD methodology can represent a potential 
      tool for investigation of M. tuberculosis gene expression.
CI  - Copyright 1999 Academic Press.
FAU - Rindi, L
AU  - Rindi L
AD  - Biotecnologie Mediche, Infettivologia ed Epidemiologia, Universita di Pisa, Pisa, 
      I-56127, Italy.
FAU - Lari, N
AU  - Lari N
FAU - Garzelli, C
AU  - Garzelli C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - *Genes, Bacterial
MH  - Mycobacterium tuberculosis/genetics/*pathogenicity
MH  - RNA, Messenger/*genetics
MH  - Virulence/*genetics
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - S0006-291X(99)90591-0 [pii]
AID - 10.1006/bbrc.1999.0591 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Apr 29;258(1):94-101. doi: 
      10.1006/bbrc.1999.0591.