PMID- 10223319
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 66
IP  - 1
DP  - 1999 Mar 31
TI  - Effect of porcine reproductive and respiratory syndrome virus (PRRSV) on alveolar 
      lung macrophage survival and function.
PG  - 15-27
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) recently emerged as 
      an important cause of reproductive disorders and pneumonia in domestic pigs 
      throughout the world. Acute cytocidal replication of PRRSV in alveolar lung 
      macrophages causes the acute pneumonia; however, it remains largely unresolved 
      whether there may also be a predisposition to longer-term local immunodeficiency 
      in the PRRSV-convalescent lung. We applied various flow cytometric techniques to 
      study the interplay between PRRSV replication and macrophage viability/function 
      in pure cultures of porcine alveolar lung macrophages. Monitored by flow 
      cytometric detection of intracellular PRRSV nucleocapsid protein, acute (24 h 
      post infection) PRRSV replication did not impede the ability of alveolar 
      macrophages to ingest fluorescently labelled Escherichia coli. At 48 h post 
      infection, PRRSV-induced cytotoxicity (quantitated by flow analysis of cell size 
      and membrane integrity) led to 40% reduction in the total number of phagocytozing 
      cells. However, viable/uninfected macrophages in PRRSV-infected cultures 
      exhibited normal phagocytic ability at 48 h, indicating that no soluble 
      phagocytosis-suppressive mediators were induced by PRRSV infection in this 
      system. In short, in our minimal system containing only a single cell type, 
      phagocytosis-suppressive effects of PRRSV infection were detected, that acted at 
      the culture level by reducing the total number of alveolar lung macrophages.
FAU - Oleksiewicz, M B
AU  - Oleksiewicz MB
AD  - Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave.
FAU - Nielsen, J
AU  - Nielsen J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 36015-30-2 (Propidium)
SB  - IM
MH  - Animals
MH  - Cell Death
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Escherichia coli
MH  - Flow Cytometry
MH  - Macrophages, Alveolar/cytology/*physiology/*virology
MH  - Nucleocapsid/analysis
MH  - Phagocytosis
MH  - Porcine Reproductive and Respiratory Syndrome/physiopathology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Propidium
MH  - Swine
MH  - Time Factors
MH  - *Virus Replication
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - S0378-1135(98)00309-5 [pii]
AID - 10.1016/s0378-1135(98)00309-5 [doi]
PST - ppublish
SO  - Vet Microbiol. 1999 Mar 31;66(1):15-27. doi: 10.1016/s0378-1135(98)00309-5.