PMID- 10223548
OWN - NLM
STAT- MEDLINE
DCOM- 19990629
LR  - 20190831
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 58
IP  - 1
DP  - 1999 May
TI  - Induction of apoptosis in cord blood lymphocytes by HHV-6.
PG  - 63-8
AB  - Apoptosis induced by human herpesvirus 6 (HHV-6) in cord blood lymphocytes was 
      investigated. Cord blood mononuclear cells (CBMC) prestimulated with 
      phytohemagglutinin (PHA) were infected with HHV-6 and cultured with interleukin 2 
      (IL-2) for 5 days. Apoptosis was investigated by cell cycle analysis, terminal 
      deoxytransferase (TdT)-mediated dUTP nick end-labeling (TUNEL) assay, and 
      staining with monoclonal antibody APO2.7 reacting with 7A6 antigen. The 
      percentage of the hypodiploid fraction by cell cycle analysis and the percentage 
      of apoptosis determined by TUNEL assay were significantly higher in 
      HHV-6-infected CBMC compared with uninfected CBMC. 7A6 antigen, induced on the 
      mitochondria membrane in apoptotic cells, were mainly expressed in CD4+ cells. 
      7A6 antigen was also detected in HHV-6-infected cells determined by monoclonal 
      antibody OHV-3 reacting with HHV-6 glycoprotein. These data indicated that HHV-6 
      induced apoptosis in HHV-6-infected cells after stimulation with IL-2 for 5 days. 
      The addition of anti-Fas antibody, anti-Fas ligand antibody, and anti-TNF-alpha 
      antibody did not affect the induction of apoptosis by HHV-6, indicating that the 
      Fas-Fas ligand pathway and TNF pathway did not contribute to the apoptosis 
      induced by HHV-6.
FAU - Ichimi, R
AU  - Ichimi R
AD  - Department of Pediatrics, Mie University School of Medicine, Tsu, Japan.
FAU - Jin-no, T
AU  - Jin-no T
FAU - Ito, M
AU  - Ito M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Antigens, Viral)
RN  - 0 (FASLG protein, human)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (antigen 7A6)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Antigens, Viral/immunology
MH  - *Apoptosis
MH  - CD4-Positive T-Lymphocytes/cytology/virology
MH  - Cell Culture Techniques/methods
MH  - Cells, Cultured
MH  - Fas Ligand Protein
MH  - Fetal Blood/cytology
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - Lymphocytes/cytology/*virology
MH  - Membrane Glycoproteins/metabolism
MH  - Membrane Proteins/biosynthesis
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - fas Receptor/metabolism
EDAT- 1999/05/01 02:12
MHDA- 2000/06/20 09:00
CRDT- 1999/05/01 02:12
PHST- 1999/05/01 02:12 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/05/01 02:12 [entrez]
AID - 10.1002/(SICI)1096-9071(199905)58:1<63::AID-JMV10>3.0.CO;2-C [pii]
AID - 10.1002/(sici)1096-9071(199905)58:1<63::aid-jmv10>3.0.co;2-c [doi]
PST - ppublish
SO  - J Med Virol. 1999 May;58(1):63-8. doi: 
      10.1002/(sici)1096-9071(199905)58:1<63::aid-jmv10>3.0.co;2-c.