PMID- 10223709
OWN - NLM
STAT- MEDLINE
DCOM- 19990609
LR  - 20191103
IS  - 0012-1592 (Print)
IS  - 0012-1592 (Linking)
VI  - 41
IP  - 2
DP  - 1999 Apr
TI  - Mosaic analysis with oep mutant reveals a repressive interaction between 
      floor-plate and non-floor-plate mutant cells in the zebrafish neural tube.
PG  - 135-42
AB  - The floor plate is located at the ventral midline of the neural tube in 
      vertebrates. Floor-plate development is severely impaired in zebrafish one-eyed 
      pinhead (oep) mutants. oep encodes a membrane-bound protein with an epiblast 
      growth factor (EGF) motif and functions autonomously in floor-plate precursors. 
      To understand the cell behavior and cell-cell interaction during floor-plate 
      development, the distribution and gene expression of wild-type and oep mutant 
      cells in genetic mosaics were examined. When mutant shield cells were 
      transplanted into a wild-type host, an ectopic neural tube with a floor plate was 
      induced. However, the floor plate of the secondary axis was consistently devoid 
      of mutant cells while its notochord was composed entirely of mutant cells. This 
      indicates that oep shield cells adopt only a notochord fate in a wild-type 
      environment. In reciprocal transplants (wild to oep), however, grafted shield 
      cells frequently contributed to part of the floor-plate region of the secondary 
      neural tube and expressed floor-plate markers. Careful examination of serial 
      sections revealed that a mutant neural cell, when located next to the wild-type 
      cells at the ventral midline, inhibited floor-plate differentiation of the 
      adjacent wild-type cells. This inhibition was effective over an area only one- or 
      two-cells wide along the anteroposterior axis. As the cells located at the 
      ventral midline of the oep neural tube are thought to possess a neural character, 
      similar to those located on either side of the floor plate in a wild-type embryo, 
      this inhibition may play an important role during normal development in 
      restricting the floor-plate region into the ventral-most midline by antagonizing 
      homeogenetic signals from the floor-plate cells.
FAU - Shinya, M
AU  - Shinya M
AD  - Division of Biological Science, Graduate School of Science, Nagoya University, 
      Japan.
FAU - Furutani-Seiki, M
AU  - Furutani-Seiki M
FAU - Kuroiwa, A
AU  - Kuroiwa A
FAU - Takeda, H
AU  - Takeda H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Dev Growth Differ
JT  - Development, growth & differentiation
JID - 0356504
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (tdgf1 protein, zebrafish)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Central Nervous System/cytology/*embryology
MH  - Epidermal Growth Factor/*genetics
MH  - Homeodomain Proteins/*genetics
MH  - *Mosaicism
MH  - *Mutation
MH  - Transcription Factors/*genetics
MH  - Zebrafish/*embryology
MH  - *Zebrafish Proteins
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - 10.1046/j.1440-169x.1999.00417.x [doi]
PST - ppublish
SO  - Dev Growth Differ. 1999 Apr;41(2):135-42. doi: 10.1046/j.1440-169x.1999.00417.x.