PMID- 10224228
OWN - NLM
STAT- MEDLINE
DCOM- 19990525
LR  - 20190516
IS  - 0892-6638 (Print)
IS  - 0892-6638 (Linking)
VI  - 13
IP  - 8
DP  - 1999 May
TI  - Calcium-dependent immunoglobulin E recognition of the apo- and calcium-bound form 
      of a cross-reactive two EF-hand timothy grass pollen allergen, Phl p 7.
PG  - 843-56
AB  - Type I allergy, an immunodisorder that affects almost 20% of the population 
      worldwide, is based on the immunoglobulin E (IgE) recognition of per se innocuous 
      antigens (allergens). Pollen from wind-pollinated plants belong to the most 
      potent allergen sources. We report the isolation of a cDNA coding for a 8.6 kDa 
      two EF-hand calcium binding allergen, Phl p 7, from a timothy grass (Phleum 
      pratense) pollen expression cDNA library, using serum IgE from a grass pollen 
      allergic patient. Sequence analysis identified Phl p 7 as a member of a recently 
      discovered subfamily of pollen-specific calcium binding proteins. Recombinant Phl 
      p 7 was expressed in Escherichia coli and purified to homogeneity as determined 
      by mass spectroscopy. Approximately 10% of pollen allergic patients displayed IgE 
      reactivity to rPhl p 7 and Phl p 7-homologous allergens present in pollens of 
      monocotyledonic and dicotyledonic plants. Circular dichroism analysis of the 
      calcium-bound and apo-rPhl p 7 indicated that differences in IgE recognition may 
      be due to calcium-induced changes in the protein conformation. The fact that 
      patients mount IgE antibodies against different protein conformations is 
      interpreted as a footprint of a preferential sensitization against either form. 
      The biological activity of rPhl p 7 was demonstrated by its ability to induce 
      basophil histamine release and immediate type skin reactions in sensitized 
      individuals. In conclusion, IgE binding to Phl p 7 represents an example for the 
      conformation-dependent IgE recognition of an allergen. Recombinant Phl p 7 may be 
      used for diagnosis and perhaps treatment of a group of patients who suffer from 
      allergy to pollens of many unrelated plant species.
FAU - Niederberger, V
AU  - Niederberger V
AD  - Department of Otorhinolaryngology, Institute of Medical and Chemical Laboratory 
      Diagnostics, University of Vienna, Austria.
FAU - Hayek, B
AU  - Hayek B
FAU - Vrtala, S
AU  - Vrtala S
FAU - Laffer, S
AU  - Laffer S
FAU - Twardosz, A
AU  - Twardosz A
FAU - Vangelista, L
AU  - Vangelista L
FAU - Sperr, W R
AU  - Sperr WR
FAU - Valent, P
AU  - Valent P
FAU - Rumpold, H
AU  - Rumpold H
FAU - Kraft, D
AU  - Kraft D
FAU - Ehrenberger, K
AU  - Ehrenberger K
FAU - Valenta, R
AU  - Valenta R
FAU - Spitzauer, S
AU  - Spitzauer S
LA  - eng
SI  - GENBANK/Y17835
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Allergens)
RN  - 0 (Apoproteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Plant)
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Allergens/genetics/*immunology/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoproteins/genetics/immunology/metabolism
MH  - Base Sequence
MH  - Calcium/*metabolism
MH  - Cross Reactions
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics/isolation & purification
MH  - DNA, Plant/genetics/isolation & purification
MH  - Escherichia coli/genetics
MH  - Histamine Release
MH  - Humans
MH  - Hypersensitivity, Immediate/etiology
MH  - Immunoglobulin E/*metabolism
MH  - In Vitro Techniques
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Plant Proteins/genetics/immunology/metabolism
MH  - Poaceae/genetics/immunology
MH  - Pollen/genetics/*immunology/*metabolism
MH  - Protein Conformation
MH  - Rabbits
MH  - Recombinant Proteins/genetics/immunology/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/04 00:00
MHDA- 1999/05/04 00:01
CRDT- 1999/05/04 00:00
PHST- 1999/05/04 00:00 [pubmed]
PHST- 1999/05/04 00:01 [medline]
PHST- 1999/05/04 00:00 [entrez]
AID - 10.1096/fasebj.13.8.843 [doi]
PST - ppublish
SO  - FASEB J. 1999 May;13(8):843-56. doi: 10.1096/fasebj.13.8.843.