PMID- 10224291
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 189
IP  - 9
DP  - 1999 May 3
TI  - Humoral and cell-mediated autoimmune reactions to human acidic ribosomal P2 
      protein in individuals sensitized to Aspergillus fumigatus P2 protein.
PG  - 1507-12
AB  - A panel of cDNAs encoding allergenic proteins was isolated from an Aspergillus 
      fumigatus cDNA library displayed on the surface of filamentous phage. Solid 
      phase-immobilized serum immunoglobulin E (IgE) from A. fumigatus-allergic 
      individuals was used to enrich phage displaying IgE-binding molecules. One of the 
      cDNAs encoded a 11.1-kD protein that was identified as acidic ribosomal 
      phosphoprotein type 2 (P2 protein). The allergen, formally termed rAsp f 8, 
      shares >62% sequence identity and >84% sequence homology to corresponding 
      eukaryotic P2 proteins, including human P2 protein. The sequences encoding human 
      and fungal P2 protein were subcloned, expressed in Escherichia coli as 
      His6-tagged fusion proteins, and purified by Ni2+-chelate affinity 
      chromatography. Both recombinant P2 proteins were recognized by IgE antibodies 
      from allergic individuals sensitized to the A. fumigatus P2 protein and elicited 
      strong type 1-specific skin reactions in these individuals. Moreover, human and 
      fungal P2 proteins induced proliferative responses in peripheral blood 
      mononuclear cells of A. fumigatus- allergic subjects sensitized to the fungal P2 
      protein. These data provide strong evidence for in vitro and in vivo humoral and 
      cell-mediated autoreactivity to human P2 protein in patients suffering from 
      chronic A. fumigatus allergy.
FAU - Mayer, C
AU  - Mayer C
AD  - Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, 
      Switzerland.
FAU - Appenzeller, U
AU  - Appenzeller U
FAU - Seelbach, H
AU  - Seelbach H
FAU - Achatz, G
AU  - Achatz G
FAU - Oberkofler, H
AU  - Oberkofler H
FAU - Breitenbach, M
AU  - Breitenbach M
FAU - Blaser, K
AU  - Blaser K
FAU - Crameri, R
AU  - Crameri R
LA  - eng
SI  - GENBANK/AJ224333
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Allergens)
RN  - 0 (DNA, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ribosomal Proteins)
RN  - 0 (phosphoprotein P2, ribosomal)
SB  - IM
MH  - Allergens/*immunology
MH  - Amino Acid Sequence
MH  - Antibody Formation
MH  - Aspergillus fumigatus/genetics/*immunology
MH  - Autoimmunity/*immunology
MH  - Base Sequence
MH  - DNA, Fungal
MH  - Fungal Proteins/genetics/*immunology
MH  - Humans
MH  - Immunity, Cellular
MH  - Leukocytes, Mononuclear/immunology
MH  - Molecular Sequence Data
MH  - Phosphoproteins/genetics/*immunology
MH  - Recombinant Fusion Proteins/biosynthesis/genetics/immunology
MH  - Ribosomal Proteins/genetics/*immunology
MH  - Sequence Homology, Amino Acid
PMC - PMC2193053
EDAT- 1999/05/04 00:00
MHDA- 1999/05/04 00:01
PMCR- 1999/11/03
CRDT- 1999/05/04 00:00
PHST- 1999/05/04 00:00 [pubmed]
PHST- 1999/05/04 00:01 [medline]
PHST- 1999/05/04 00:00 [entrez]
PHST- 1999/11/03 00:00 [pmc-release]
AID - 10.1084/jem.189.9.1507 [doi]
PST - ppublish
SO  - J Exp Med. 1999 May 3;189(9):1507-12. doi: 10.1084/jem.189.9.1507.