PMID- 10225885
OWN - NLM
STAT- MEDLINE
DCOM- 19990518
LR  - 20200724
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 67
IP  - 5
DP  - 1999 May
TI  - Conformational and linear B-cell epitopes of Asp f 2, a major allergen of 
      Aspergillus fumigatus, bind differently to immunoglobulin E antibody in the sera 
      of allergic bronchopulmonary aspergillosis patients.
PG  - 2284-91
AB  - Asp f 2 is a major Aspergillus fumigatus allergen involved in allergic 
      bronchopulmonary aspergillosis. Knowledge of the B-cell epitopes may contribute 
      to the understanding of immunoregulation and immunodiagnosis. To elucidate the 
      immunoglobulin E (IgE) binding epitopes in the linear sequence of Asp f 2, we 
      synthesized decamer peptides spanning the whole molecule of Asp f 2 on 
      derivatized cellulose membranes and evaluated IgE binding in ABPA patient and 
      control sera. Peptides three to five amino acids long were synthesized based on 
      amino acid sequences within the IgE binding regions and evaluated for the 
      specificity of epitope antibody interactions. Nine IgE binding regions were 
      recognized in this protein of 268 amino acid residues. Of the nine epitopes, 
      seven (ATQRRQI, RKYFG, HWR, YTTRR, DHFAD, ALEAYA, and THEGGQ) are present in the 
      hydrophilic regions of Asp f 2. Immunologic evaluation of the three recombinant 
      fragments, Asp f 2A encompassing the N-terminal epitope region, Asp f 2B without 
      N- and C-terminal regions of the protein, and Asp f 2C representing C-terminal 
      epitopes, revealed that either the N- or C-terminal region of the protein is 
      essential for the correct folding and conformation for IgE antibody binding.
FAU - Banerjee, B
AU  - Banerjee B
AD  - Department of Medicine, Allergy-Immunology Division, Medical College of 
      Wisconsin, Milwaukee, Wisconsin 53226, USA.
FAU - Greenberger, P A
AU  - Greenberger PA
FAU - Fink, J N
AU  - Fink JN
FAU - Kurup, V P
AU  - Kurup VP
LA  - eng
GR  - AI42349/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (DNA Primers)
RN  - 0 (Epitopes)
RN  - 0 (Fungal Proteins)
RN  - 0 (allergen Asp f 2, Aspergillus fumigatus)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*chemistry/genetics
MH  - Amino Acid Sequence
MH  - Antibodies, Fungal/blood
MH  - Antibody Specificity
MH  - Antigens, Fungal/*chemistry/genetics
MH  - Aspergillosis, Allergic Bronchopulmonary/*immunology
MH  - Aspergillus fumigatus/genetics/*immunology
MH  - B-Lymphocytes/*immunology
MH  - Base Sequence
MH  - Case-Control Studies
MH  - DNA Primers/genetics
MH  - Epitopes/chemistry/genetics
MH  - Fungal Proteins/*chemistry/genetics/*immunology
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Molecular Sequence Data
MH  - Protein Conformation
PMC - PMC115968
EDAT- 1999/05/04 00:00
MHDA- 1999/05/04 00:01
PMCR- 1999/05/01
CRDT- 1999/05/04 00:00
PHST- 1999/05/04 00:00 [pubmed]
PHST- 1999/05/04 00:01 [medline]
PHST- 1999/05/04 00:00 [entrez]
PHST- 1999/05/01 00:00 [pmc-release]
AID - 0030 [pii]
AID - 10.1128/IAI.67.5.2284-2291.1999 [doi]
PST - ppublish
SO  - Infect Immun. 1999 May;67(5):2284-91. doi: 10.1128/IAI.67.5.2284-2291.1999.