PMID- 10225970
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20240109
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 103
IP  - 9
DP  - 1999 May
TI  - Chronic inflammation upregulates chemokine receptors and induces neutrophil 
      migration to monocyte chemoattractant protein-1.
PG  - 1269-76
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that stimulates 
      monocyte recruitment when injected into tissues of healthy animals. However, the 
      function of this chemokine in models with preexisting inflammation is not known. 
      Therefore, MCP-1 was superfused over the mesentery of naive rats or rats with 
      chronic adjuvant-induced vasculitis. MCP-1 elicited increased leukocyte 
      transendothelial migration in adjuvant-immunized rats compared with naive 
      animals. Surprisingly, histology revealed that neutrophils constituted the 
      majority of leukocytes recruited in adjuvant-immunized animals. In vitro, MCP-1 
      was also able to induce chemotaxis of neutrophils isolated from 
      adjuvant-immunized rats but not from naive rats. Flow cytometry revealed novel 
      expression of the CC chemokine receptors CCR1 and CCR2 on neutrophils from 
      adjuvant-immunized animals. In naive animals, an antibody against CD18 blocked 
      leukocyte adhesion and emigration in response to MCP-1. In adjuvant-immunized 
      animals, leukocyte adhesion was reduced by antibodies against the alpha4-integrin 
      but not by antibodies against CD18. However, the CD18 antibody did block 
      emigration. To our knowledge, this study is the first to show increased 
      sensitivity to a CC chemokine in a model with preexisting inflammation, and 
      altered leukocyte recruitment profiles in response to MCP-1. It also demonstrates 
      that CD18 is required for chemokine-induced leukocyte transendothelial migration, 
      independent of its known role in mediating firm adhesion. J. Clin. Invest. 
      103:1269-1276 (1999).
FAU - Johnston, B
AU  - Johnston B
AD  - Immunology Research Group, Department of Physiology and Biophysics, University of 
      Calgary, Calgary, Alberta T2N 4N1, Canada.
FAU - Burns, A R
AU  - Burns AR
FAU - Suematsu, M
AU  - Suematsu M
FAU - Issekutz, T B
AU  - Issekutz TB
FAU - Woodman, R C
AU  - Woodman RC
FAU - Kubes, P
AU  - Kubes P
LA  - eng
GR  - P01 HL042550/HL/NHLBI NIH HHS/United States
GR  - HL-42550/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Movement
MH  - Chemokine CCL2/*metabolism
MH  - Chronic Disease
MH  - Male
MH  - Neutrophils/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Chemokine/*metabolism
MH  - *Up-Regulation
MH  - Vasculitis/*metabolism
PMC - PMC408354
EDAT- 1999/05/04 00:00
MHDA- 1999/05/04 00:01
PMCR- 1999/05/01
CRDT- 1999/05/04 00:00
PHST- 1999/05/04 00:00 [pubmed]
PHST- 1999/05/04 00:01 [medline]
PHST- 1999/05/04 00:00 [entrez]
PHST- 1999/05/01 00:00 [pmc-release]
AID - 05208 [pii]
AID - 10.1172/JCI5208 [doi]
PST - ppublish
SO  - J Clin Invest. 1999 May;103(9):1269-76. doi: 10.1172/JCI5208.