PMID- 10226150
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20190513
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 517 ( Pt 1)
IP  - Pt 1
DP  - 1999 May 15
TI  - Novel regulation of the A-type K+ current in murine proximal colon by 
      calcium-calmodulin-dependent protein kinase II.
PG  - 75-84
AB  - 1. The kinetics of inactivation of delayed rectifier K+ current in murine colonic 
      myocytes differed in amphotericin-permeabilized patch and conventional patch 
      clamp. The difference was accounted for by Ca2+ buffering. 2. 
      Calcium-calmodulin-dependent protein kinase II (CaMKII) inhibitors increased the 
      rate of inactivation and slowed recovery from inactivation of the outward 
      current. This was seen in single steps and in the envelope of the current tails. 
      The effect was largely on the TEA-insensitive component of current. 3. Dialysis 
      of myocytes with autothiophosphorylated CaMKII slowed inactivation. This effect 
      was reversed by addition of CaMKII inhibitor. 4. Antibodies revealed CaMKII-like 
      immunoreactivity in murine colonic myocytes and other cells. Immunoblots 
      identified a small protein with CaMKII-like immunoreactivity in homogenates of 
      colonic muscle. 5. We conclude that CaMKII regulates delayed rectifier K+ 
      currents in murine colonic myocytes. The changes in the delayed rectifier current 
      may participate in the Ca2+-dependent regulation of gastrointestinal motility.
FAU - Koh, S D
AU  - Koh SD
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine, Reno, NV 89557, USA. skoh@physio.unr.edu
FAU - Perrino, B A
AU  - Perrino BA
FAU - Hatton, W J
AU  - Hatton WJ
FAU - Kenyon, J L
AU  - Kenyon JL
FAU - Sanders, K M
AU  - Sanders KM
LA  - eng
GR  - P01 DK041315/DK/NIDDK NIH HHS/United States
GR  - DK41315/DK/NIDDK NIH HHS/United States
GR  - NS36318/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Benzylamines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Sulfonamides)
RN  - 139298-40-1 (KN 93)
RN  - 63HM46XPOW (KN 62)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - RWP5GA015D (Potassium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/analogs & derivatives/pharmacology
MH  - Animals
MH  - Benzylamines/pharmacology
MH  - Calcium/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Colon/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Gastrointestinal Motility/physiology
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Muscle, Smooth/metabolism
MH  - Patch-Clamp Techniques
MH  - Potassium/*metabolism
MH  - Sulfonamides/pharmacology
PMC - PMC2269312
EDAT- 1999/05/05 00:00
MHDA- 1999/05/05 00:01
PMCR- 2000/05/15
CRDT- 1999/05/05 00:00
PHST- 1999/05/05 00:00 [pubmed]
PHST- 1999/05/05 00:01 [medline]
PHST- 1999/05/05 00:00 [entrez]
PHST- 2000/05/15 00:00 [pmc-release]
AID - 10.1111/j.1469-7793.1999.0075z.x [doi]
PST - ppublish
SO  - J Physiol. 1999 May 15;517 ( Pt 1)(Pt 1):75-84. doi: 
      10.1111/j.1469-7793.1999.0075z.x.