PMID- 10226518
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20061115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 19
IP  - 1A
DP  - 1999 Jan-Feb
TI  - Comparison of comparative genomic hybridization, fluorescence in situ 
      hybridization and flow cytometry in urinary bladder cancer.
PG  - 7-12
AB  - Comparative genomic hybridization (CGH) was applied to screen the genetic events 
      in six invasive urinary bladder cancers. These cases were also studied by flow 
      cytometry (FCM) and fluorescence in situ hybridization (FISH). Four samples 
      showed partial gain on chromosome 8, with the common region involved was on 
      8q23-qter. Full or partial deletion on chromosome 2 and 17p in addition to gain 
      on 20q was found in two cases. Interestingly one diploid tumor with low mitotic 
      index, stage and grade showed more genetic aberrations (8 gains and 7 losses) by 
      CGH than other aneuploid tumors with high mitotic index, stage and grade. The 
      numerical chromosomal aberration detected by FISH for chromosomes 7, 8, 9, 10, 11 
      and 17 were 50% in T1 cases and 100% in T2-T4 cases. FISH was performed on 
      chromosome 8q and 17p to compare and validate the sensitivity of CGH. The 
      agreement was 100% for 8q24 locus and 50% for p53 locus. This indicates that 
      different molecular genetic techniques showed relatively different aspect of 
      genomic aberrations.
FAU - Mahdy, E
AU  - Mahdy E
AD  - Department of Pathology, University Hospital, Uppsala, Sweden.
FAU - Yoshihiro, S
AU  - Yoshihiro S
FAU - Zech, L
AU  - Zech L
FAU - Wester, K
AU  - Wester K
FAU - Pan, Y
AU  - Pan Y
FAU - Busch, C
AU  - Busch C
FAU - Dohner, H
AU  - Dohner H
FAU - Kallioniemi, O
AU  - Kallioniemi O
FAU - Bergerheim, U
AU  - Bergerheim U
FAU - Malmstrom, P U
AU  - Malmstrom PU
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/*analysis
MH  - *Flow Cytometry
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Nucleic Acid Hybridization
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 1999/05/05 00:00
MHDA- 1999/05/05 00:01
CRDT- 1999/05/05 00:00
PHST- 1999/05/05 00:00 [pubmed]
PHST- 1999/05/05 00:01 [medline]
PHST- 1999/05/05 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1999 Jan-Feb;19(1A):7-12.