PMID- 10226590
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20131121
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 19
IP  - 1A
DP  - 1999 Jan-Feb
TI  - Hepatocyte growth factor/scatter factor disrupts epithelial tumour cell-cell 
      adhesion: involvement of beta-catenin.
PG  - 509-17
AB  - Initial events in the metastatic spread of tumours involve loss of cell-cell 
      adhesion within the primary tumour mass. The integrity and morphology of 
      epithelial tumour cell colonies is maintained primarily by cell-cell adhesions 
      mediated by E-cadherin and its associated intracellular catenin molecules. 
      Hepatocyte growth factor/scatter factor (HGF/SF) is a potent promoter of the 
      metastatic functions of tumour cells, including motility and invasion and also 
      induces the dissociation of tumour cell colonies. In this study we report that 
      HGF/SF promoted the scattering of an epithelial colorectal tumour cell line. 
      Western blotting demonstrated that this was not due to a change in level of 
      either E-cadherin or its associated catenin molecules. Immunoprecipitation 
      studies revealed that HGF/SF elevated the level of tyrosine-phosphorylated 
      beta-catenin within these cells together with reducing the amount of E-cadherin 
      that was observed to co-precipitate with the beta-catenin. These results were 
      confirmed with confocal scanning laser microscopy. We conclude that 
      phosphorylation of beta-catenin by HGF/SF affects its association with E-cadherin 
      at the cell surface and thus regulates E-cadherin function resulting in colony 
      scattering phenomena.
FAU - Hiscox, S
AU  - Hiscox S
AD  - Department of Surgery, University of Wales College of Medicine, Health Park, 
      Cardiff, U.K. Hiscox@cf.ac.uk
FAU - Jiang, W G
AU  - Jiang WG
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (beta Catenin)
RN  - 42HK56048U (Tyrosine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Cadherins/analysis/metabolism
MH  - Cell Adhesion/*drug effects
MH  - Colonic Neoplasms/*pathology
MH  - Cytoskeletal Proteins/analysis/*physiology
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Microscopy, Confocal
MH  - Phosphorylation
MH  - *Trans-Activators
MH  - Tumor Cells, Cultured
MH  - Tyrosine/metabolism
MH  - beta Catenin
EDAT- 1999/05/05 00:00
MHDA- 1999/05/05 00:01
CRDT- 1999/05/05 00:00
PHST- 1999/05/05 00:00 [pubmed]
PHST- 1999/05/05 00:01 [medline]
PHST- 1999/05/05 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1999 Jan-Feb;19(1A):509-17.