PMID- 10226596
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20141120
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 19
IP  - 1A
DP  - 1999 Jan-Feb
TI  - Cell-cell adhesion molecules and their associated proteins in bladder cancer 
      cells and their role in mitogen induced cell-cell dissociation and invasion.
PG  - 547-52
AB  - Three bladder cell lines (EJ138, RT112/84 and T24/83) were used to examine the 
      expression of E-cadherin, desmoglein, their associated proteins and their role in 
      cell-cell dissociation and invasion using hepatocyte growth factor/scatter factor 
      (HGF/SF). Both immunocytochemistry and Western blotting revealed that RT112/84 
      cells expressed high levels of E-cadherin, alpha-, beta-, and gamma-catenins. 
      However, there was no expression of E-cadherin and very low levels of alpha- and 
      beta-catenin expression detected in both EJ138 and T24/83 cells. In contrast, all 
      three cell lines were found to express desmoglein, desmoplakin and c-Met. An in 
      vitro invasion assay showed that both EJ138 and T24/83 cells were highly 
      invasive, however, RT112/84 cells were found to have very limited invasion. 
      Invasion of RT112/84 cells was significantly increased by inclusion of either 
      antibody to E-cadherin or motogen (HGF/SF) in the culture medium. This did not 
      appear to be the case for EJ138 and T24/83 cells. Using immunoprecipitation, 
      HGF/SF induced tyrosine phosphorylation of beta-catenin but not desmoplakin. It 
      is concluded that E-cadherin plays a stronger role than desmosomal cadherin in 
      the control of the in vitro invasion of bladder cancer cells. Activation of 
      beta-catenin and subsequent dysfunction of E-cadherin may be a key mechanism in 
      the induction of invasion by the motogen, HGF/SF.
FAU - Davies, G
AU  - Davies G
AD  - University Department of Surgery, University of Wales College of Medicine, Heath 
      Park, Cardiff, U.K. DAVIESG11@cf.ac.uk
FAU - Jiang, W G
AU  - Jiang WG
FAU - Mason, M D
AU  - Mason MD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (CTNNB1 protein, mouse)
RN  - 0 (Cadherins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DSP protein, human)
RN  - 0 (Desmogleins)
RN  - 0 (Desmoplakins)
RN  - 0 (Dsp protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (beta Catenin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Animals
MH  - Cadherins/*analysis
MH  - Cytoskeletal Proteins/*analysis/metabolism
MH  - Desmogleins
MH  - Desmoplakins
MH  - Hepatocyte Growth Factor/pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Mice
MH  - Neoplasm Invasiveness
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-met/analysis
MH  - *Trans-Activators
MH  - Tumor Cells, Cultured
MH  - Urinary Bladder Neoplasms/chemistry/*pathology
MH  - beta Catenin
EDAT- 1999/05/05 00:00
MHDA- 1999/05/05 00:01
CRDT- 1999/05/05 00:00
PHST- 1999/05/05 00:00 [pubmed]
PHST- 1999/05/05 00:01 [medline]
PHST- 1999/05/05 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1999 Jan-Feb;19(1A):547-52.