PMID- 10226600
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20131121
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 19
IP  - 1A
DP  - 1999 Jan-Feb
TI  - Antimutagenicity of lignin in vitro.
PG  - 569-72
AB  - The inhibitory activity of lignin against nitrosoguanidine (MNNG)- and acridine 
      orange (AO)- induced mutagenesis was examined using two microbial systems: green 
      unicellular flagellate Euglena gracilis and Salmonella typhimurium TA100 and 
      TA97. To verify the hypothesis that the above mentioned mutagens may generate 
      some oxidant species and subsequently free radicals, or they may interact with 
      lignin, two physico-chemical measurements were performed. Lignin at a tested 
      concentration (100 micrograms/ml) decreases Euglena-bleaching activity of MNNG by 
      67.7% and AO by 99.7%. Percentage of MNNG-induced revertants of S. typhimurium 
      was also decreased substantially by lignin. We conclude that our results indicate 
      the possible mechanisms behind the antimutagenic/anticarcinogenic effects of 
      lignin: namely, scavening of reactive oxygen species produced by MNNG and binding 
      of AO itself.
FAU - Ebringer, L
AU  - Ebringer L
AD  - Institute of Cell Biology, Faculty of Science, Comenius University, Bratislava, 
      Slovak Republic. ebringer@m2.fedu.uniba.sk
FAU - Krizkova, L
AU  - Krizkova L
FAU - Polonyi, J
AU  - Polonyi J
FAU - Dobias, J
AU  - Dobias J
FAU - Lahitova, N
AU  - Lahitova N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Antimutagenic Agents)
RN  - 11062-77-4 (Superoxides)
RN  - 12H3O2UGSF (Methylnitronitrosoguanidine)
RN  - 9005-53-2 (Lignin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/toxicity
MH  - Animals
MH  - Antimutagenic Agents/*pharmacology
MH  - Euglena gracilis/drug effects
MH  - Lignin/*pharmacology
MH  - Methylnitronitrosoguanidine/toxicity
MH  - Salmonella typhimurium/drug effects
MH  - Superoxides/metabolism
EDAT- 1999/05/05 00:00
MHDA- 1999/05/05 00:01
CRDT- 1999/05/05 00:00
PHST- 1999/05/05 00:00 [pubmed]
PHST- 1999/05/05 00:01 [medline]
PHST- 1999/05/05 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1999 Jan-Feb;19(1A):569-72.