PMID- 10227120
OWN - NLM
STAT- MEDLINE
DCOM- 19990628
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 66
IP  - 3
DP  - 1999 Apr 19
TI  - Monoclonal antibodies to the GP5 of porcine reproductive and respiratory syndrome 
      virus are more effective in virus neutralization than monoclonal antibodies to 
      the GP4.
PG  - 171-86
AB  - The arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) 
      contains six structural proteins the roles of which are not completely 
      understood. In a preceding study, immunization with the dutch isolate I10 of 
      PRRSV had led to the development of MAbs against four structural proteins 
      [Wieczorek-Krohmer, M., 1994. Herstellung und Charakterisierung von monoklonalen 
      Antikorpern gegen das Virus des Porzinen Reproduktiven und Respiratorischen 
      Syndroms (PRRSV). Inaugural-Dissertation, Ludwig-Maximilians-Universitat, 
      Munchen] here finally identified by reaction with individual plasmid-expressed 
      PRRSV proteins as products of ORFs 3 (GP3), 4 (GP4), 5 (GP5) and 7 (N). 
      Surprisingly, the MAbs against GP5 revealed the presence of two antigenically 
      distinct virus populations in the isolate I10, the population PRRSV-'PPV', 
      isolated from plaques and the PRRSV-'EPV', gained by end point dilution. MAbs 
      against GP3, GP4 and N reacted with both I10 populations as well as with natural 
      PRRSV isolates. However, the anti-GP5 MAbs exclusively recognized PRRSV-'PPV'. In 
      this study immunization of mice with both separated I10 populations confirmed 
      that solely PRRSV-'PPV' possesses the property to induce an immune response 
      ultimately leading to the establishment of MAbs against GP5. Whereas the 15 
      anti-GP5 MAbs (derived from four independent fusions) reacted exclusively with 
      PRRSV-'PPV' of the isolate I10, anti-GP4 MAbs detected their target antigen on 
      various isolates of European origin and were able to neutralize them. As 
      indicated by competition assays and selection of neutralization-resistant virus 
      mutants, all GP5 MAbs are directed against a single antigenic site on the ORF 5 
      protein. Both groups of neutralizing antibodies bound to the surface of purified 
      virions demonstrating that the recognized epitopes represent surface structures 
      of the virion envelope. However, anti-GP5 MAbs mediated the binding of more gold 
      granules than anti-GP4 MAbs. Comparison of the neutralizing effect of anti-GP4 
      and anti-GP5 MAbs revealed the anti-GP5 MAbs as the more efficient antibodies. 
      For the complete neutralization of about 100 ID50 of PRRSV-'PPV' anti-GP5 culture 
      supernatant was effective up to a dilution of 1:1280 whereas the most effective 
      anti-GP4 antibodies exhibited a comparable effect only up to 1:64. These results 
      indicate that PRRSV GP5 in principle is a major target for neutralizing 
      antibodies, as is found for other arteriviruses, but that in nature 'ORF 5 escape 
      mutants' may develop as easily as in vitro.
FAU - Weiland, E
AU  - Weiland E
AD  - Federal Research Centre for Virus Diseases of Animals, Tubingen, Germany. 
      emilie.weiland@tue.bfav.de
FAU - Wieczorek-Krohmer, M
AU  - Wieczorek-Krohmer M
FAU - Kohl, D
AU  - Kohl D
FAU - Conzelmann, K K
AU  - Conzelmann KK
FAU - Weiland, F
AU  - Weiland F
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Structural Proteins)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/immunology
MH  - Blotting, Western/veterinary
MH  - Cell Line
MH  - DNA, Viral/chemistry
MH  - Fluorescein-5-isothiocyanate/chemistry
MH  - Fluorescent Antibody Technique, Indirect/veterinary
MH  - Glycoproteins/genetics/*immunology
MH  - Hominidae
MH  - Macrophages, Alveolar/cytology/immunology
MH  - Mice
MH  - Microscopy, Immunoelectron/veterinary
MH  - Neutralization Tests/veterinary
MH  - Open Reading Frames
MH  - Plasmids/chemistry
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - Transfection
MH  - Viral Structural Proteins/genetics/*immunology
EDAT- 1999/05/05 00:00
MHDA- 1999/05/05 00:01
CRDT- 1999/05/05 00:00
PHST- 1999/05/05 00:00 [pubmed]
PHST- 1999/05/05 00:01 [medline]
PHST- 1999/05/05 00:00 [entrez]
AID - S0378-1135(99)00006-1 [pii]
AID - 10.1016/s0378-1135(99)00006-1 [doi]
PST - ppublish
SO  - Vet Microbiol. 1999 Apr 19;66(3):171-86. doi: 10.1016/s0378-1135(99)00006-1.