PMID- 10227295
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20061115
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 398
IP  - 6729
DP  - 1999 Apr 22
TI  - MCP-1 and IL-8 trigger firm adhesion of monocytes to vascular endothelium under 
      flow conditions.
PG  - 718-23
AB  - Monocytes contribute to the development of atherosclerotic lesions in mouse 
      models. The chemoattractant proteins (chemokines), monocyte chemoattractant 
      protein-1 (MCP-1) and interleukin-8 (IL-8), are found in human atheroma, and mice 
      lacking receptors for these chemokines are less susceptible to atherosclerosis 
      and have fewer monocytes in vascular lesions. Although MCP-1 has a powerful 
      effect on monocytes, IL-8 is thought to act predominantly on neutrophils and it 
      is unclear how it could recruit monocytes. Here we investigate the ability of 
      chemokines to control the interaction of monocytes under flow conditions with 
      vascular endothelium that has been transduced to express specific 
      leukocyte-adherence receptors. We find that MCP-1 and IL-8 can each rapidly cause 
      rolling monocytes to adhere firmly onto monolayers expressing E-selectin, whereas 
      related chemokines do not. These effects do not correlate with either the 
      induction of a calcium transient or chemotaxis. We conclude that chemokines are 
      important modulators of monocyte-endothelial interactions under flow conditions. 
      Moreover, our finding that IL-8 is a powerful trigger for firm adhesion of 
      monocytes to vascular endothelium reveals an unexpected role for this chemokine 
      in monocyte recruitment.
FAU - Gerszten, R E
AU  - Gerszten RE
AD  - The Cardiovascular Research Center and Cardiology Division, Massachusetts General 
      Hospital, Harvard Medical School, Boston 02114, USA.
FAU - Garcia-Zepeda, E A
AU  - Garcia-Zepeda EA
FAU - Lim, Y C
AU  - Lim YC
FAU - Yoshida, M
AU  - Yoshida M
FAU - Ding, H A
AU  - Ding HA
FAU - Gimbrone, M A Jr
AU  - Gimbrone MA Jr
FAU - Luster, A D
AU  - Luster AD
FAU - Luscinskas, F W
AU  - Luscinskas FW
FAU - Rosenzweig, A
AU  - Rosenzweig A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Chemokine CCL2)
RN  - 0 (E-Selectin)
RN  - 0 (Interleukin-8)
RN  - 0 (Receptors, Leukocyte-Adhesion)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
SB  - IM
MH  - Cell Adhesion/physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*physiology
MH  - Chemotaxis, Leukocyte/*physiology
MH  - E-Selectin/physiology
MH  - Endothelium, Vascular/*physiology
MH  - Gene Transfer Techniques
MH  - Humans
MH  - Interleukin-8/*physiology
MH  - Monocytes/*physiology
MH  - Receptors, Leukocyte-Adhesion/physiology
MH  - Umbilical Veins/cytology
MH  - Vascular Cell Adhesion Molecule-1/genetics/physiology
EDAT- 1999/05/05 02:03
MHDA- 2001/03/23 10:01
CRDT- 1999/05/05 02:03
PHST- 1999/05/05 02:03 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/05/05 02:03 [entrez]
AID - 10.1038/19546 [doi]
PST - ppublish
SO  - Nature. 1999 Apr 22;398(6729):718-23. doi: 10.1038/19546.