PMID- 10227806
OWN - NLM
STAT- MEDLINE
DCOM- 19990528
LR  - 20181201
IS  - 1521-6616 (Print)
IS  - 1521-6616 (Linking)
VI  - 91
IP  - 2
DP  - 1999 May
TI  - Neutrophilic myeloperoxidase-macrophage interactions perpetuate chronic 
      inflammation associated with experimental arthritis.
PG  - 145-55
AB  - Rheumatoid arthritis is a systemic disease of unknown etiology. The purpose of 
      this study was to elucidate an unrecognized interaction between neutrophilic 
      myeloperoxidase (MPO) and macrophages (Mphi) which could perpetuate the 
      inflammatory response associated with arthritis. A monoarticular arthritis was 
      induced by intra-articular injection of group A streptococcus cell wall fragments 
      (PG-APS) into the ankle joint of female Lewis rats. After swelling/erythema 
      subsided, joints were reinjected with either recombinant MPO or enzymatically 
      inactive MPO (iMPO). Joint measurements were made daily and arthritis was 
      confirmed by histology. Neither iMPO nor MPO could initiate "clinical" arthritis; 
      however, either form of the enzyme injected after PG-APS induced a dose-dependent 
      increase in erythema and swelling. Mannans, which block the binding of MPO to Mo, 
      ablated clinical symptoms. Also, the presence of tumor necrosis factor alpha was 
      observed only in diseased joints using immunocytochemistry.
CI  - Copyright 1999 Academic Press.
FAU - Lefkowitz, D L
AU  - Lefkowitz DL
AD  - Texas Tech University, Lubbock, Texas 79409, USA.
FAU - Gelderman, M P
AU  - Gelderman MP
FAU - Fuhrmann, S R
AU  - Fuhrmann SR
FAU - Graham, S
AU  - Graham S
FAU - Starnes, J D 3rd
AU  - Starnes JD 3rd
FAU - Lefkowitz, S S
AU  - Lefkowitz SS
FAU - Bollen, A
AU  - Bollen A
FAU - Moguilevsky, N
AU  - Moguilevsky N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - Arthritis, Rheumatoid/*immunology/pathology
MH  - Disease Models, Animal
MH  - Female
MH  - Macrophages/*immunology
MH  - Neutrophils/*enzymology
MH  - Peroxidase/*immunology
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Streptococcus pyogenes/immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 1999/05/05 02:04
MHDA- 2001/03/28 10:01
CRDT- 1999/05/05 02:04
PHST- 1999/05/05 02:04 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/05/05 02:04 [entrez]
AID - S1521-6616(99)94696-X [pii]
AID - 10.1006/clim.1999.4696 [doi]
PST - ppublish
SO  - Clin Immunol. 1999 May;91(2):145-55. doi: 10.1006/clim.1999.4696.