PMID- 10228019
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20071115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 9
DP  - 1999 May 1
TI  - Down-regulation of CXCR4 by human herpesvirus 6 (HHV-6) and HHV-7.
PG  - 5417-22
AB  - Recent studies have demonstrated that human herpesvirus 6 (HHV-6) and HHV-7 
      interact with HIV-1 and alter the expression of various surface molecules and 
      functions of T lymphocytes. The present study was undertaken to clarify whether 
      coreceptors for HIV-1, CXCR4 and CCR5, are necessary for HHV-6 and HHV-7 
      infection. Although CXCR4 and CCR5 appeared not to be the coreceptors for these 
      viruses, marked down-regulation of CXCR4, but not CCR5, was detected in HHV-6 
      variant A (HHV-6A)-, HHV-6 variant B (HHV-6B)-, and HHV-7-infected cells. 
      Down-regulation of CXCR4 resulted in impairment of chemotaxis and a decreased 
      level of elevation of the intracellular Ca2+ concentration in response to stromal 
      cell-derived factor-1. Northern blot analysis of mRNAs extracted from HHV-6A-, 
      HHV-6B-, and HHV-7-infected CD4+ T lymphocytes demonstrated a markedly decreased 
      level of CXCR4 gene transcription, but the posttranscriptional stability of CXCR4 
      mRNA was not significantly altered. These data demonstrate that unlike HIV-1, 
      HHV-6 and HHV-7 infections do not require expression of CXCR4 or CCR5, whereas 
      marked down-regulation of CXCR4 is induced by these viruses, suggesting that 
      HHV-6 and HHV-7 infections may render CD4+ T lymphocytes resistant to T 
      lymphocyte-tropic HIV-1 infection.
FAU - Yasukawa, M
AU  - Yasukawa M
AD  - First Department of Internal Medicine, Ehime University School of Medicine, 
      Japan. yasukawa@m.ehime-u.ac.jp
FAU - Hasegawa, A
AU  - Hasegawa A
FAU - Sakai, I
AU  - Sakai I
FAU - Ohminami, H
AU  - Ohminami H
FAU - Arai, J
AU  - Arai J
FAU - Kaneko, S
AU  - Kaneko S
FAU - Yakushijin, Y
AU  - Yakushijin Y
FAU - Maeyama, K
AU  - Maeyama K
FAU - Nakashima, H
AU  - Nakashima H
FAU - Arakaki, R
AU  - Arakaki R
FAU - Fujita, S
AU  - Fujita S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CXC)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Antibodies, Monoclonal/pharmacology
MH  - CD4-Positive T-Lymphocytes/immunology/metabolism/virology
MH  - Cell Movement/immunology
MH  - Cells, Cultured
MH  - Chemokine CXCL12
MH  - Chemokines, CXC/physiology
MH  - Down-Regulation/*immunology
MH  - Herpesvirus 6, Human/*physiology
MH  - Herpesvirus 7, Human/*physiology
MH  - Humans
MH  - RNA, Messenger/antagonists & inhibitors/biosynthesis
MH  - Receptors, CXCR4/*antagonists & inhibitors/genetics/immunology
MH  - Virus Replication/immunology
EDAT- 1999/05/05 00:00
MHDA- 1999/05/05 00:01
CRDT- 1999/05/05 00:00
PHST- 1999/05/05 00:00 [pubmed]
PHST- 1999/05/05 00:01 [medline]
PHST- 1999/05/05 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 May 1;162(9):5417-22.