PMID- 10229918
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 14
IP  - 2
DP  - 1999 Mar
TI  - Detection of aneuploidy by multicolor FISH in mouse sperm after in vivo treatment 
      with acrylamide, colchicine, diazepam or thiabendazole.
PG  - 173-9
AB  - Multicolor fluorescence in situ hybridization (FISH) was used to investigate the 
      induction of aneuploidy during meiosis in young adult male mice treated with 
      chemicals chosen for the EU sponsored aneuploidy project (acrylamide, colchicine, 
      diazepam and thiabendazole). The aim of the present study was to evaluate the 
      frequency of aneuploid sperm induced by each of these chemicals by sperm FISH. 
      Male (102/ElxC3H/El)F1 mice were treated with acrylamide (120 and 60 mg/kg single 
      dose i.p.), colchicine (1.5 and 3 mg/kg single dose, i.p.), diazepam (300, 150 
      and 75 mg/kg single dose by oral intubation) or thiabendazole (100 and 300 mg/kg 
      daily for 11 days by oral intubation). At 22 days after the last treatment, sperm 
      were collected from the cauda epididymis. Three chromosome FISH was applied to 
      determine hyperhaploid and diploid sperm with DNA probes specific for the 
      chromosomes X, Y and 8. Five animals were treated per dose group and sperm 
      aneuploidy was evaluated in 10,000 sperm per animal. We found significant 
      increases in the frequency of total hyperhaploidy for the males treated with 3.0 
      mg/kg colchicine (0.092 versus 0.056%, P < 0.05) and with 1.5 mg/kg colchicine 
      (0.082 versus 0.050%, P < 0.05), as well for the males treated with 300 mg/kg 
      diazepam (0.081 versus 0.050%, P < 0.05), indicating that colchicine and diazepam 
      each induced germ cell aneuploidy. We also found significant increases in the 
      frequency of total diploidy for the males treated with 300 mg/kg diazepam (P < 
      0.05) and with 300 mg/kg thiabendazole (P < 0.05). No significant effects were 
      found for 120 and 60 mg/kg acrylamide or for the other doses of diazepam and 
      thiabendazole. These first results indicate that the multicolor FISH method is 
      useful to determine aneuploidy induction in sperm of mice.
FAU - Schmid, T E
AU  - Schmid TE
AD  - Institut fur Saugetiergenetik, GSF-Forschungszentrum fur Umwelt, Neuherberg, 
      Germany.
FAU - Xu, W
AU  - Xu W
FAU - Adler, I D
AU  - Adler ID
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 20R035KLCI (Acrylamide)
RN  - N1Q45E87DT (Thiabendazole)
RN  - Q3JTX2Q7TU (Diazepam)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Acrylamide/*pharmacology
MH  - *Aneuploidy
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Chromosome Aberrations/chemically induced
MH  - Chromosome Disorders
MH  - Colchicine/*pharmacology
MH  - Diazepam/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Spermatozoa/*drug effects/metabolism
MH  - Thiabendazole/*pharmacology
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
AID - 10.1093/mutage/14.2.173 [doi]
PST - ppublish
SO  - Mutagenesis. 1999 Mar;14(2):173-9. doi: 10.1093/mutage/14.2.173.