PMID- 10231027 OWN - NLM STAT- MEDLINE DCOM- 19990720 LR - 20190513 IS - 1340-2838 (Print) IS - 1340-2838 (Linking) VI - 6 IP - 1 DP - 1999 Feb 26 TI - Isolation and mapping of a putative b subunit of human ATP synthase (ATP-BL) from human leukocytes. PG - 29-35 AB - From a human-leukocyte cDNA library, we cloned cDNA encoding a novel protein, which has a significant homology with the b subunit of ATP synthase (proton-transporting ATPase, F1F0-ATPase; EC3.6.1.34) derived from Anabaena sp. strain PCC 7120. The cDNA has an open reading frame of 1314 nucleotides corresponding to 438 amino acids. The coding sequence was 37.9% identical over 57 amino acid with b subunit of ATP synthase. The 34-amino-acid region of the predicted peptide sequence displays a coiled-coil motif that could form a complex with some other protein(s). We designated this novel gene as ATP-BL because of its homology to the b subunit of ATP synthase. The ATP-BL locus was mapped by fluorescence in situ hybridization (FISH) and radiation hybrid mapping to the q24 region of chromosome 16. FAU - Sugimoto, J AU - Sugimoto J AD - Department of Materials and Biosystem Engineering, Faculty of Engineering, Toyama University, Toyama City, Japan. FAU - Hatakeyama, T AU - Hatakeyama T FAU - Isobe, M AU - Isobe M LA - eng SI - GENBANK/AB018551 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - DNA Res JT - DNA research : an international journal for rapid publication of reports on genes and genomes JID - 9423827 RN - EC 3.6.3.14 (Proton-Translocating ATPases) SB - IM MH - Amino Acid Sequence MH - Base Sequence MH - Blotting, Northern MH - Chromosome Banding MH - Chromosome Mapping MH - Chromosomes, Human, Pair 16 MH - Cloning, Molecular MH - Humans MH - In Situ Hybridization, Fluorescence MH - Leukocytes/*enzymology MH - Molecular Sequence Data MH - Physical Chromosome Mapping MH - Proton-Translocating ATPases/*genetics/physiology MH - Sequence Homology, Amino Acid MH - Tissue Distribution EDAT- 1999/05/07 00:00 MHDA- 1999/05/07 00:01 CRDT- 1999/05/07 00:00 PHST- 1999/05/07 00:00 [pubmed] PHST- 1999/05/07 00:01 [medline] PHST- 1999/05/07 00:00 [entrez] AID - 10.1093/dnares/6.1.29 [doi] PST - ppublish SO - DNA Res. 1999 Feb 26;6(1):29-35. doi: 10.1093/dnares/6.1.29.