PMID- 10231961
OWN - NLM
STAT- MEDLINE
DCOM- 19990528
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 42
IP  - 2
DP  - 1999 Apr
TI  - FISH landmarks for barley chromosomes (Hordeum vulgare L.).
PG  - 274-81
AB  - Barley metaphase chromosomes (2n = 14) can be identified by fluorescence in situ 
      hybridization (FISH) and digital imaging microscopy using heterologous 18S rDNA 
      and 5S rDNA probe sequences. When these sequences are used together, FISH 
      landmark signals were seen so that all 7 chromosomes were uniquely identified and 
      unambiguously oriented. The chromosomal location of the landmark signals was 
      determined by FISH to a barley trisomic series using the 18S and 5S probes 
      labeled with different fluorophores. The utility of these FISH landmarks for 
      barley physical mapping was also demonstrated when an Amy-2 cDNA clone and a BAC 
      clone were hybridized with the FISH landmark probes.
FAU - Brown, S E
AU  - Brown SE
AD  - Department of Bioagricultural Sciences and Pest Management, College of 
      Agricultural Sciences, Colorado State University, Fort Collins 80523, USA. 
      sbrown@ops.agsci.colostate.edu
FAU - Stephens, J L
AU  - Stephens JL
FAU - Lapitan, N L
AU  - Lapitan NL
FAU - Knudson, D L
AU  - Knudson DL
LA  - eng
GR  - AI34337/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Aedes/genetics
MH  - Animals
MH  - Chromosome Mapping
MH  - DNA Probes
MH  - *DNA, Plant
MH  - *DNA, Ribosomal
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *RNA, Ribosomal, 18S
MH  - *RNA, Ribosomal, 5S
EDAT- 1999/05/08 00:00
MHDA- 1999/05/08 00:01
CRDT- 1999/05/08 00:00
PHST- 1999/05/08 00:00 [pubmed]
PHST- 1999/05/08 00:01 [medline]
PHST- 1999/05/08 00:00 [entrez]
PST - ppublish
SO  - Genome. 1999 Apr;42(2):274-81.