PMID- 10232421
OWN - NLM
STAT- MEDLINE
DCOM- 19990629
LR  - 20190822
IS  - 0903-1936 (Print)
IS  - 0903-1936 (Linking)
VI  - 13
IP  - 3
DP  - 1999 Mar
TI  - Establishment of four new mesothelioma cell lines: characterization by 
      ultrastructural and immunophenotypic analysis.
PG  - 527-34
AB  - The aim of this study was to assess the biological characteristics of four new 
      malignant mesothelioma (MM) cell lines. Since simian virus (SV)40 sequences have 
      been recently detected in MM, SV40 large T antigen (Tag) expression was also 
      analysed. MM cell lines were characterized by morphological, ultrastructural and 
      cytogenetic analysis. Expression of Tag and of relevant MM markers was studied by 
      immunocytochemistry, surface antigens by indirect immunofluorescence and 
      immunomodulating cytokines by enzyme-linked immunosorbent assay (ELISA). The four 
      MM cell lines, established from pleural effusions, showed a slow proliferation 
      rate and pleomorphic changes during culture. Cell lines expressed vimentin, 
      cytokeratins 8 and 18, and the mesothelial antigen recognized by HBME-1 
      monoclonal antibody, but not carcinoembryonic antigen. Surface human leukocyte 
      antigen (HLA)-class I and intercellular adhesion molecule (ICAM)-1 molecules were 
      present on all the cell lines. While HLA class II and CD86 were constitutively 
      undetectable, HLA-class II was present after interferon (IFN)-gamma stimulation. 
      All cell lines displayed abnormal karyotypes with chromosome 6 abnormalities. 
      Transforming growth factor (TGF)-beta2 and interleukin (IL)-6 were constitutively 
      secreted, while tumour necrosis factor (TNF)-alpha was secreted only in response 
      to lipopolysaccharide. Intranuclear Tag was expressed in two cell lines. The 
      persistence of large T antigen with human leukocyte antigen class I and 
      intercellular adhesion molecule-1 positivity may point to large T antigen as a 
      target for cytotoxic T-lymphocyte-based immunotherapy in some malignant 
      mesothelioma patients.
FAU - Orengo, A M
AU  - Orengo AM
AD  - Istituto Nazionale per la Ricerca sul Cancro, Centro di Biotechnologie Avanzate, 
      Genoa, Italy.
FAU - Spoletini, L
AU  - Spoletini L
FAU - Procopio, A
AU  - Procopio A
FAU - Favoni, R E
AU  - Favoni RE
FAU - De Cupis, A
AU  - De Cupis A
FAU - Ardizzoni, A
AU  - Ardizzoni A
FAU - Castagneto, B
AU  - Castagneto B
FAU - Ribotta, M
AU  - Ribotta M
FAU - Betta, P G
AU  - Betta PG
FAU - Ferrini, S
AU  - Ferrini S
FAU - Mutti, L
AU  - Mutti L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur Respir J
JT  - The European respiratory journal
JID - 8803460
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cytokines)
SB  - IM
MH  - Antigens, Differentiation, T-Lymphocyte/analysis
MH  - Biomarkers, Tumor/*analysis
MH  - Cytokines/analysis
MH  - Diagnosis, Differential
MH  - Female
MH  - Fluorometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Male
MH  - Mesothelioma/*chemistry/*ultrastructure
MH  - Pleural Effusion, Malignant/chemistry/*cytology
MH  - Pleural Neoplasms/*chemistry/*ultrastructure
MH  - Sensitivity and Specificity
MH  - Tumor Cells, Cultured/chemistry/ultrastructure
EDAT- 1999/05/08 00:00
MHDA- 1999/05/08 00:01
CRDT- 1999/05/08 00:00
PHST- 1999/05/08 00:00 [pubmed]
PHST- 1999/05/08 00:01 [medline]
PHST- 1999/05/08 00:00 [entrez]
AID - 10.1183/09031936.99.13352799 [doi]
PST - ppublish
SO  - Eur Respir J. 1999 Mar;13(3):527-34. doi: 10.1183/09031936.99.13352799.