PMID- 10232541
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20220223
IS  - 0148-396X (Print)
IS  - 0148-396X (Linking)
VI  - 44
IP  - 5
DP  - 1999 May
TI  - In vitro modulation of microglia motility by glioma cells is mediated by 
      hepatocyte growth factor/scatter factor.
PG  - 1077-82; discussion 1082-3
AB  - OBJECTIVE: Considered as immune effector cells of the central nervous system, 
      microglia represent a major component of the inflammatory cells found in 
      malignant gliomas. Although their role in brain tumor biology is unclear, 
      accumulation of microglia in malignant brain tumors may be mediated through 
      active secretion of cytokines by glioma cells. Because hepatocyte growth 
      factor/scatter factor (HGF/SF) has been shown to modulate glioma motility through 
      an autocrine mechanism, and because microglia have been reported to express the 
      HGF/SF receptor Met, we hypothesized that microglia recruitment by gliomas may 
      also occur through the secretion of HGF/SF. METHODS: The effect of glioma cells 
      in augmenting BV-2 murine microglia motility was studied by using an in vitro 
      Boyden chamber migration assay. To determine the chemokines involved in microglia 
      migration, neutralizing monoclonal antibodies against monocyte chemotactic 
      protein-1 and HGF/SF were tested. Immunoblotting was used to check for the 
      expression of HGF/SF by glioma cells, and the expression of Met by BV-2 cells was 
      examined by flow cytometry. RESULTS: BV-2 migration was noted within 7 hours of 
      incubation with both human (U251 MG and U373 MG) and murine (GL261) glioma cell 
      lines. This migration corresponded to HGF/SF secretion by glioma cells and was 
      completely inhibited by neutralizing monoclonal antibody against HGF/SF, but not 
      monocyte chemotactic protein-1. Exposure of BV-2 cells to recombinant HGF/SF, but 
      not monocyte chemotactic protein-1, resulted in their migration and 
      down-regulation of Met in a dose-dependent fashion. CONCLUSION: HGF/SF, which 
      plays a role in glioma motility and mitogenesis, may also act as a chemokine for 
      microglia and may be responsible for the microglia infiltration in malignant 
      gliomas. This active recruitment of microglia may play an important role in 
      glioma biology.
FAU - Badie, B
AU  - Badie B
AD  - Department of Neurological Surgery, University of Wisconsin School of Medicine, 
      Madison, USA.
FAU - Schartner, J
AU  - Schartner J
FAU - Klaver, J
AU  - Klaver J
FAU - Vorpahl, J
AU  - Vorpahl J
LA  - eng
GR  - P30 CA14520/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neurosurgery
JT  - Neurosurgery
JID - 7802914
RN  - 0 (Recombinant Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Animals
MH  - Brain Neoplasms/metabolism/pathology/*physiopathology
MH  - Cell Movement/physiology
MH  - Glioma/metabolism/pathology/*physiopathology
MH  - Hepatocyte Growth Factor/biosynthesis/*physiology
MH  - Humans
MH  - Mice
MH  - Microglia/*physiology
MH  - Proto-Oncogene Proteins c-met/metabolism
MH  - Recombinant Proteins
MH  - Tumor Cells, Cultured
EDAT- 1999/05/08 00:00
MHDA- 1999/05/08 00:01
CRDT- 1999/05/08 00:00
PHST- 1999/05/08 00:00 [pubmed]
PHST- 1999/05/08 00:01 [medline]
PHST- 1999/05/08 00:00 [entrez]
AID - 10.1097/00006123-199905000-00075 [doi]
PST - ppublish
SO  - Neurosurgery. 1999 May;44(5):1077-82; discussion 1082-3. doi: 
      10.1097/00006123-199905000-00075.