PMID- 10233397
OWN - NLM
STAT- MEDLINE
DCOM- 19990610
LR  - 20041117
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 105
IP  - 1
DP  - 1999 Apr
TI  - Monitoring of human herpesviruses after allogeneic peripheral blood stem cell 
      transplantation and bone marrow transplantation.
PG  - 295-302
AB  - Herpesviruses frequently cause serious complications after allogeneic bone marrow 
      transplantation (allo-BMT). Recent studies have shown more rapid immune 
      reconstitution after allogeneic peripheral blood stem cell transplantation 
      (allo-PBSCT) compared with allo-BMT. However, it has not been clarified whether 
      the improved immune reconstitution after allo-PBSCT is associated with a lower 
      incidence of herpesvirus infections. We monitored the emergence of Epstein-Barr 
      virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and HHV-7 DNA by 
      a nested-double polymerase chain reaction in peripheral blood leucocytes from 22 
      allo-BMT and 16 allo-PBSCT patients. Each virus had an unique temporal profile of 
      detection. HHV-6 DNA was detected most frequently at 3 weeks after 
      transplantation, whereas CMV and EBV DNA were detected later (2-3 months). 
      Detection rates of HHV-6 DNA at 3 and 4 weeks after allo-BMT were significantly 
      higher than those after allo-PBSCT (9/16 v 2/13 at 3 weeks, P < 0.01; 10/21 v 
      1/15 at 4 weeks, P < 0.01). Detection rates of the other three herpesviruses 
      after the two types of allogeneic transplantation were not significantly 
      different throughout observation period. Furthermore, detection of HHV-6 DNA 
      within the first 4 weeks was associated with delayed platelet engraftment after 
      both allo-BMT and allo-PBSCT (P < 0.01). These results suggest an advantage for 
      allo-PBSCT over allo-BMT in terms of suppression of HHV-6 reactivation and 
      prevention of subsequent complications.
FAU - Maeda, Y
AU  - Maeda Y
AD  - Second Department of Internal Medicine, Okayama University Medical School, 
      Okayama, Japan.
FAU - Teshima, T
AU  - Teshima T
FAU - Yamada, M
AU  - Yamada M
FAU - Shinagawa, K
AU  - Shinagawa K
FAU - Nakao, S
AU  - Nakao S
FAU - Ohno, Y
AU  - Ohno Y
FAU - Kojima, K
AU  - Kojima K
FAU - Hara, M
AU  - Hara M
FAU - Nagafuji, K
AU  - Nagafuji K
FAU - Hayashi, S
AU  - Hayashi S
FAU - Fukuda, S
AU  - Fukuda S
FAU - Sawada, H
AU  - Sawada H
FAU - Matsue, K
AU  - Matsue K
FAU - Takenaka, K
AU  - Takenaka K
FAU - Ishimaru, F
AU  - Ishimaru F
FAU - Ikeda, K
AU  - Ikeda K
FAU - Niiya, K
AU  - Niiya K
FAU - Harada, M
AU  - Harada M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Marrow Transplantation/*methods
MH  - Child
MH  - Cytomegalovirus/isolation & purification
MH  - DNA, Viral/analysis
MH  - Female
MH  - Graft Survival
MH  - Graft vs Host Disease/virology
MH  - Hematologic Diseases/therapy/virology
MH  - Hematopoietic Stem Cell Transplantation/*methods
MH  - Herpesvirus 4, Human/*isolation & purification
MH  - Herpesvirus 6, Human/*isolation & purification
MH  - Herpesvirus 7, Human/*isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Transplantation, Homologous
EDAT- 1999/05/08 00:00
MHDA- 1999/05/08 00:01
CRDT- 1999/05/08 00:00
PHST- 1999/05/08 00:00 [pubmed]
PHST- 1999/05/08 00:01 [medline]
PHST- 1999/05/08 00:00 [entrez]
PST - ppublish
SO  - Br J Haematol. 1999 Apr;105(1):295-302.