PMID- 10233706
OWN - NLM
STAT- MEDLINE
DCOM- 19990716
LR  - 20231105
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 96
IP  - 2
DP  - 1999 Feb
TI  - Immunoglobulin E suppression and cytokine modulation in mice orally tolerized to 
      beta-lactoglobulin.
PG  - 278-85
AB  - This study was designed to confirm the tolerogenic properties of 
      beta-lactoglobulin in a mouse model and to assess specific oral tolerance 
      induction in humoral and cellular compartments. BALB/c mice were fed 
      beta-lactoglobulin (BLG) or whey proteins at different ages and subsequently 
      intraperitoneally challenged 5 days later with both BLG and a non-specific 
      antigen, ovalbumin (OVA). Three weeks later, oral tolerance induction was 
      analysed in CMP-fed, versus saline-fed mice, by measuring specific seric and 
      intestinal antibody responses, delayed-type hypersensitivity (DTH), specific 
      splenocyte proliferation, and cytokine secretion patterns. Three-week-old mice 
      fed high doses of either whey proteins or BLG (respectively 3 mg/g or 5 mg/g of 
      body weight) were found to achieve oral tolerization. At humoral and mucosal 
      levels, anti-BLG immunoglobulin E (IgE) were suppressed in these groups when 
      compared with saline fed mice. With respect to cellular responses, systemic DTH 
      and lymphocyte proliferation to BLG were also inhibited in CMP-fed mice. Weaning 
      time was determined to be the best period for oral tolerance induction. Kinetic 
      analyses showed however, that a minimum of 2 weeks was required for oral 
      tolerance detection. Finally, cytokine profiles indicated a reciprocal decrease 
      of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) versus an increase of 
      IL-10 and transforming growth factor-beta (TGF-beta) secretions in tolerized 
      mice. Taken together, these results clearly showed that oral administration of 
      high doses of cows' milk proteins can induce significant hyposensitization in 
      mice, in a specific inhibition of T helper 1 (Th1) lymphocytes with the 
      participation of suppressor cytokines.
FAU - Pecquet, S
AU  - Pecquet S
AD  - Food Immunology, Nestec SA, Nestle Research Centre Lausanne, Switzerland.
FAU - Pfeifer, A
AU  - Pfeifer A
FAU - Gauldie, S
AU  - Gauldie S
FAU - Fritsche, R
AU  - Fritsche R
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-2)
RN  - 0 (Lactoglobulins)
RN  - 0 (Milk Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Age Factors
MH  - Allergens/*administration & dosage/immunology
MH  - Analysis of Variance
MH  - Animals
MH  - Antibody Formation
MH  - Cytokines/*immunology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - *Immune Tolerance
MH  - Immunity, Mucosal
MH  - Immunoglobulin E/*immunology
MH  - Interferon-gamma/analysis
MH  - Interleukin-10/analysis
MH  - Interleukin-2/analysis
MH  - Lactoglobulins/*administration & dosage/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Milk Proteins/administration & dosage/immunology
MH  - Th1 Cells/immunology
MH  - Transforming Growth Factor beta/analysis
PMC - PMC2326731
EDAT- 1999/05/08 00:00
MHDA- 1999/05/08 00:01
PMCR- 2000/02/01
CRDT- 1999/05/08 00:00
PHST- 1999/05/08 00:00 [pubmed]
PHST- 1999/05/08 00:01 [medline]
PHST- 1999/05/08 00:00 [entrez]
PHST- 2000/02/01 00:00 [pmc-release]
AID - imm678 [pii]
AID - 10.1046/j.1365-2567.1999.00678.x [doi]
PST - ppublish
SO  - Immunology. 1999 Feb;96(2):278-85. doi: 10.1046/j.1365-2567.1999.00678.x.