PMID- 10233740
OWN - NLM
STAT- MEDLINE
DCOM- 19990730
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 96
IP  - 4
DP  - 1999 Apr
TI  - Surface and gene expression of immunoglobulin E receptors on mast cells and 
      mast-cell numbers in interleukin-4-gene knockout mice.
PG  - 544-50
AB  - We quantified immunoglobulin E (IgE) on peritoneal mast cells of interleukin-4 
      (IL-4)-gene knockout (-/-) mice and wild-type (+/+) controls using a 
      cytofluorometric method, and examined the expression of IgE receptors, estimated 
      by quantifying the total binding of IgE on the mast cells of IL-4 (-/-) mice. The 
      mast cells of IL-4 (+/+) mice, identified and measured using microscope 
      fluorometry, had a fluorescence intensity five to six times higher than that of 
      non-mast cells, while the mast cells obtained from IL-4 (-/-) mice had 
      fluorescence intensities within the range of those of non-mast cells. Two weeks 
      after an infection with Nippostrongylus brasiliensis, the fluorescence intensity 
      of the mast cells of IL-4 (+/+) mice increased to a level about twice as high as 
      that before immunization. However, no significant increase after infection was 
      observed in IL-4 (-/-) mice. Furthermore, the mast cells of IL-4 (-/-) mice did 
      not bind IgE when incubated with IgE at concentrations that saturated IgE 
      receptors on the mast cells of wild-type controls, thereby indicating that the 
      expression of IgE receptors on mast cells was impaired in the IL-4-deficient 
      mice. Using a reverse transcription-polymerase chain reaction, we found gene 
      expression of all three subunits (alpha-, beta- and gamma-chains) of the IgE 
      receptor in IL-4 (-/-) like that in IL-4 (+/+) mice. The results thus suggest 
      that the binding of IgE may be essential to induce the translation of mRNA to 
      IgE-receptor proteins. We also observed that there were about twice as many 
      peritoneal mast cells in the IL-4 (-/-) mice as there were in the IL-4 (+/+) 
      mice, in both immunized and non-immunized animals. This was unexpected in view of 
      previous findings suggesting that IL-4, in concert with stem cell factor and 
      IL-3, stimulates the proliferation and differentiation of mast cells in vitro.
FAU - Chen, X J
AU  - Chen XJ
AD  - Department of Pathology, Institute of Laboratory Medicine, Sahlgrenska University 
      Hospital, Goteborg, Sweden.
FAU - Lycke, N
AU  - Lycke N
FAU - Enerback, L
AU  - Enerback L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, IgE)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Gene Expression/immunology
MH  - Immunoglobulin E/metabolism
MH  - Interleukin-4/genetics/*immunology
MH  - Male
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nippostrongylus
MH  - Peritoneal Cavity/cytology
MH  - RNA, Messenger/genetics
MH  - Receptors, IgE/genetics/*metabolism
MH  - Strongylida Infections/immunology
PMC - PMC2326780
EDAT- 1999/05/08 00:00
MHDA- 1999/05/08 00:01
PMCR- 2000/04/01
CRDT- 1999/05/08 00:00
PHST- 1999/05/08 00:00 [pubmed]
PHST- 1999/05/08 00:01 [medline]
PHST- 1999/05/08 00:00 [entrez]
PHST- 2000/04/01 00:00 [pmc-release]
AID - imm727 [pii]
AID - 10.1046/j.1365-2567.1999.00727.x [doi]
PST - ppublish
SO  - Immunology. 1999 Apr;96(4):544-50. doi: 10.1046/j.1365-2567.1999.00727.x.