PMID- 10234453
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20191103
IS  - 1385-299X (Print)
IS  - 1385-299X (Linking)
VI  - 4
IP  - 1
DP  - 1999 Apr
TI  - Temporally structured impulse activity in spontaneously discharging somatosensory 
      cortical neurons in the awake cat: recognition and quantitative description of 
      four different patterns of bursts, post-recording GFAP immunohistology and 
      computer reconstruction of the studied cortical surface.
PG  - 49-68
AB  - We elaborated two methods used in two previous publications [J. Martinson, H.H. 
      Webster, A.A. Myasnikov, R.W. Dykes, Recognition of temporally structured 
      activity in spontaneously discharging neurons in the somatosensory cortex in 
      waking cats, Brain Res. 750 (1997) 129-140 [16]; H.H. Webster, I. Salimi, A.A. 
      Myasnikov, R.W. Dykes. The effects of peripheral deafferentation on spontaneously 
      bursting neurons in the somatosensory cortex of waking cats, Brain Res. 750 
      (1997) 109-121 [21]]: (A) a procedure for detecting and classifying brief epochs 
      of high-frequency extracellular impulse activity (bursts) recorded chronically in 
      the somatosensory cortex of the awake cat, and (B) a modification of an 
      immunohistochemical technique [L.A. Bevento, L.B. McCleary. An immunochemical 
      method for marking microelectrode tracks following single-unit recordings in long 
      surviving, awake monkeys, J. Neurosci. Meth. 41 (1992) 199-204 [5]] for 
      visualization of electrode tracks and electrolytic lesions around the tip of 
      tungsten-in-glass microelectrodes [D.M.D. Landis, The early reactions of 
      non-neuronal cells to brain injury, Annu. Rev. Neurosci. 17 (1994) 133-151 [15]] 
      weeks after lesions were made in cortex. The burst recognition and classification 
      method uses an interval threshold to determine the beginning and end of one epoch 
      [M. Armstrong-James, K. Fox, Effects of ionophoresed noradrenaline on spontaneous 
      activity of neurons in rat primary somatosensory cortex, J. Physiol. (London), 
      335 (1983) 427-447 [3]] in the original sequence of interspike intervals (ISIs) 
      to segregate and analyze separately a burst. The threshold is based on the 
      duration of the shortest modal ISI found in the autocorrelogram [J. Martinson, 
      H.H. Webster, A.A. Myasnikov, R.W. Dykes, Recognition of temporally structured 
      activity in spontaneously discharging neurons in the somatosensory cortex in 
      waking cats, Brain Res. 750 (1997) 129-140 [16]]. The technique allowed 
      recognition of bursts with several distinctive patterns: (i) an initial, longer 
      ISI followed by progressively shorter ones; (ii) an initially shorter ISI 
      followed by progressively longer ones; (iii) patterns where the intermediate ISI 
      could be either longer or shorter than surrounding ones; and (iv) consecutive 
      ISIs of relatively equal duration. Among the cells discharging in bursts with 
      equal ISIs, the technique allows recognition of cells generating only short (up 
      to three to five intervals) bursts, and others generating mixtures of a short and 
      long (up to six or more intervals) bursts. Finally, frequency distributions of 
      the probability of encountering bursts having intervals of a stated length is 
      described. The visualization of tracks from chronic recording experiments is 
      important for relating neuronal function to a specific cytoarchitectural region 
      and a specific cortical layer. Several modifications of the procedure of 
      immunostaining for GFAP allows identification of recording sites in clearer 
      relationship to the cytoarchitectonic details of cat somatosensory cortex.
FAU - Miasnikov, A A
AU  - Miasnikov AA
AD  - Departement de physiologie, Centre de recherche en sciences neurologiques de 
      Montreal, Universite de Montreal, Quebec, Canada.
FAU - Webster, H H
AU  - Webster HH
FAU - Dykes, R W
AU  - Dykes RW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Brain Res Protoc
JT  - Brain research. Brain research protocols
JID - 9716650
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Animals
MH  - Cats
MH  - Differential Threshold
MH  - Electrophysiology
MH  - Glial Fibrillary Acidic Protein/*metabolism
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Male
MH  - Neurons/metabolism/*physiology
MH  - Reaction Time/physiology
MH  - Somatosensory Cortex/cytology/metabolism/*physiology
EDAT- 1999/05/11 00:00
MHDA- 1999/05/11 00:01
CRDT- 1999/05/11 00:00
PHST- 1999/05/11 00:00 [pubmed]
PHST- 1999/05/11 00:01 [medline]
PHST- 1999/05/11 00:00 [entrez]
AID - S1385-299X(99)00004-5 [pii]
AID - 10.1016/s1385-299x(99)00004-5 [doi]
PST - ppublish
SO  - Brain Res Brain Res Protoc. 1999 Apr;4(1):49-68. doi: 
      10.1016/s1385-299x(99)00004-5.