PMID- 10319612
OWN - NLM
STAT- MEDLINE
DCOM- 19990521
LR  - 20190921
IS  - 0023-5679 (Print)
IS  - 0023-5679 (Linking)
VI  - 46
IP  - 1
DP  - 1999
TI  - Kinetics of tissue and serum matrix metalloproteinase-3 and tissue inhibitor of 
      metalloproteinases-1 in intervertebral disc degeneration and disc herniation.
PG  - 39-50
AB  - We have evaluated the correlation between disc herniation or degeneration and the 
      levels of matrix metalloproteinase-3 (MMP-3) or tissue inhibitor of 
      metalloproteinases-1 (TIMP-1). The lumbar discs obtained from 22 autopsied 
      cadavers and 21 patients with lumbar disc herniation were examined. 
      Immunohistochemical study: The number of MMP-3-producing and TIMP-1-producing 
      cells increased as disc herniation or degeneration progressed. Zymography: 
      Activated type MMP-3 was observed in the normal and herniated intervertebral disc 
      excluding the intervertebral disc with protrusion-type herniation. RT-PCR: The 
      MMP-3 gene was expressed in all groups. However, the expression of the TIMP-1 
      gene was weak in the normal group as well as in the protrusion type disc 
      herniation. Determination of tissue and serum levels of MMP-3 and TIMP-1 was 
      increased as disc degeneration or herniation progressed. There were positive 
      correlations between the number of MMP-3-producing or TIMP-1-producing cells in 
      the intervertebral disc and the tissue or serum levels of MMP-3 and TIMP-1. These 
      results suggested that MMP-3 and TIMP-1 were closely related to the progression 
      of disc degeneration or herniation. The results also suggested that MMP-3 and 
      TIMP-1 exhibited similar kinetics. Moreover, the serum levels of MMP-3 and TIMP-1 
      reflected the progression of disc degeneration and herniation.
FAU - Nishida, T
AU  - Nishida T
AD  - Department of Anatomy, Kurume University School of Medicine, Japan.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Kurume Med J
JT  - The Kurume medical journal
JID - 2985210R
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Female
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Immunohistochemistry
MH  - Intervertebral Disc Displacement/*metabolism
MH  - Kinetics
MH  - Male
MH  - Matrix Metalloproteinase 3/*biosynthesis
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinase-1/*biosynthesis
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.2739/kurumemedj.46.39 [doi]
PST - ppublish
SO  - Kurume Med J. 1999;46(1):39-50. doi: 10.2739/kurumemedj.46.39.