PMID- 10320030
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20220331
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 264
IP  - 1-3
DP  - 1999 Apr 2
TI  - Sustained phosphorylation and activation of protein kinase B correlates with 
      brain-derived neurotrophic factor and insulin stimulated survival of cerebellar 
      granule cells.
PG  - 125-8
AB  - Brain-derived neurotrophic factor (BDNF) and insulin promote the survival of 6-7 
      day old post-natal rat cerebellar granule cells. Previous studies using the PI3 
      kinase inhibitor, wortmannin and the over-expression of protein kinase B (PKB) 
      have indicated that both PI3 kinase and PKB activation are central for 
      insulin-stimulated survival of these neurones. Here we report that BDNF, insulin 
      and epidermal growth factor (EGF) all cause the phosphorylation and stimulation 
      of endogenous PKB activity, though with differing profiles. The addition of BDNF, 
      or insulin resulted in a rapid and sustained phosphorylation and stimulation of 
      PKB activity, whilst EGF stimulation, which does not promote survival, caused a 
      more transient phosphorylation and stimulation of PKB activity. We also 
      investigated the involvement of the PKB substrate, glycogen synthase kinase 3 
      (GSK 3). All three growth factors caused the inactivation of GSK-3beta, 
      suggesting that the inactivation of GSK-3beta does not correlate with survival.
FAU - Foulstone, E J
AU  - Foulstone EJ
AD  - Department of Biochemistry, School of Medical Sciences, University of Bristol, 
      UK.
FAU - Tavare, J M
AU  - Tavare JM
FAU - Gunn-Moore, F J
AU  - Gunn-Moore FJ
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Insulin)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.11.- (Glycogen Synthase Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Cell Survival/drug effects
MH  - Cerebellum/cytology/*drug effects/*enzymology
MH  - Enzyme Activation/physiology
MH  - Epidermal Growth Factor/pharmacology
MH  - Glycogen Synthase Kinase 3
MH  - Glycogen Synthase Kinases
MH  - Insulin/*pharmacology
MH  - Neurons/*enzymology
MH  - *Protein Serine-Threonine Kinases
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-akt
MH  - Rats
MH  - Time Factors
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - S0304-3940(99)00166-4 [pii]
AID - 10.1016/s0304-3940(99)00166-4 [doi]
PST - ppublish
SO  - Neurosci Lett. 1999 Apr 2;264(1-3):125-8. doi: 10.1016/s0304-3940(99)00166-4.