PMID- 10320759
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20190831
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 114
IP  - 2
DP  - 1999 May 14
TI  - Forskolin-induced expression of tyrosine hydroxylase in human foetal brain 
      cortex.
PG  - 201-6
AB  - Brain-derived neurotrophic factor (BDNF) has previously been shown by this and 
      other laboratories to work in concert with dopamine (DA) to induce the 
      dopaminergic phenotype in foetal rat and human cerebral cortex during specified 
      sensitive developmental stages. In the present study this induction by BDNF/DA 
      was found to be greatly amplified by adding forskolin (fsk: 10 microM) to the rat 
      and human cerebral cortex cultures together with DA (10 microM) and BDNF (50 
      ng/ml). This amplification was 14-fold for human tissue and 2-fold for rat tissue 
      treated over an 80% shorter period. Compared to treatment with BDNF alone, the 
      additional fsk increased tyrosine hydroxylase-positive (TH+) cell numbers by 
      220-fold in the human and 26-fold in the rat tissue. Parallel reverse 
      transcription-polymerase chain reaction (RT-PCR) measurement of TH mRNA showed 
      substantial increases above control levels when BDNF/DA or BDNF/DA/fsk treatments 
      were applied. Since fsk boosts intracellular levels of cyclic AMP (cAMP), its 
      amplifying action when added together with BDNF/DA is likely to be due to 
      interactions via the cAMP response element/cAMP response element binding protein 
      (CRE/CREB) systems. This is discussed.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Pliego Rivero, F B
AU  - Pliego Rivero FB
AD  - Imperial College of Science, Technology and Medicine, Department of Biochemistry, 
      South Kensington, London SW7 2AY, UK.
FAU - McCormack, W J
AU  - McCormack WJ
FAU - Jauniaux, E
AU  - Jauniaux E
FAU - Stern, G M
AU  - Stern GM
FAU - Bradford, H F
AU  - Bradford HF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 1F7A44V6OU (Colforsin)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cells, Cultured
MH  - Cerebral Cortex/embryology/*enzymology
MH  - Colforsin/*pharmacology
MH  - DNA Primers
MH  - Dopamine/pharmacology
MH  - Embryo, Mammalian
MH  - Fetus
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Humans
MH  - Neurons/*enzymology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription, Genetic/*drug effects
MH  - Tyrosine 3-Monooxygenase/analysis/*genetics
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - S0165380699000346 [pii]
AID - 10.1016/s0165-3806(99)00034-6 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 1999 May 14;114(2):201-6. doi: 
      10.1016/s0165-3806(99)00034-6.